Liu Yuebo, Ding Chunmei, He Libang, Yang Xiao, Gou Yaping, Xu Xinyuan, Liu Yanpeng, Zhao Changsheng, Li Jianshu, Li Jiyao
State Key Laboratory of Oral Diseases National Clinical Research Center for Oral Diseases Dept. of Cariology and Endodonics West China Hospital of Stomatology, Sichuan University, China.
J Mater Chem B. 2018 Apr 7;6(13):1984-1994. doi: 10.1039/c7tb03067c. Epub 2018 Mar 16.
The regeneration of mineral crystals under physiological conditions is an efficient way to repair defects in hard tissues. To achieve robust mineralization on surfaces such as the tooth enamel, an inducer requires strong affinity with the substrates and should be able to induce mineralization. Thus far, most studies used a single molecule containing two components to realize the above functions separately, which might be troublesome to synthesize and purify. In this work, inspired by the statherin in the salivary acquired pellicle, we designed a simple peptide sequence, Asp-Asp-Asp-Glu-Glu-Lys-Cys (peptide-7), to accomplish the dual tasks of adsorption and mineralization on enamel surfaces. We speculate the calcium binding ability of the negatively charged carboxylic acid groups in the peptide itself contributes to the dual functions of peptide-7. In vitro and in vivo experiments demonstrated its excellent repair effect on enamel as compared to fluoride. More importantly, due to the strong affinity between peptides and hydroxyapatite, a compact mineralized crystal layer and a strong adhesion between the regenerated minerals and the bottom substrates were observed, similar to the effect induced by fluoride. This work sheds light on the interaction mechanism between peptide-7 and minerals. In addition, since it is safer than fluoride, peptide-7 may have potential applications in the repair of other hard tissues and the functionalization of biomaterials.
在生理条件下矿物晶体的再生是修复硬组织缺陷的有效方法。为了在牙釉质等表面实现强大的矿化作用，诱导剂需要与底物有很强的亲和力，并且应该能够诱导矿化。到目前为止，大多数研究使用含有两个组分的单一分子来分别实现上述功能，这在合成和纯化方面可能会很麻烦。在这项工作中，受唾液获得性薄膜中富含脯氨酸的蛋白质的启发，我们设计了一个简单的肽序列，天冬氨酸-天冬氨酸-天冬氨酸-谷氨酸-谷氨酸-赖氨酸-半胱氨酸（肽-7），以完成在牙釉质表面的吸附和矿化双重任务。我们推测该肽本身带负电荷的羧酸基团的钙结合能力有助于肽-7的双重功能。体外和体内实验表明，与氟化物相比，它对牙釉质具有优异的修复效果。更重要的是，由于肽与羟基磷灰石之间的强亲和力，观察到形成了致密的矿化晶体层，并且再生矿物质与底部底物之间有很强的附着力，这与氟化物诱导的效果相似。这项工作揭示了肽-7与矿物质之间的相互作用机制。此外，由于它比氟化物更安全，肽-7可能在其他硬组织的修复和生物材料的功能化方面具有潜在应用。
