Zhou Zhongzheng, Hu Fang, Hu Shihao, Kong Ming, Feng Chao, Liu Ya, Cheng Xiaojie, Ji Qiuxia, Chen Xiguang
College of Marine Life Science, Ocean University of China, 5 Yushan Road, 266003 Qingdao, China.
J Mater Chem B. 2018 Jan 28;6(4):586-592. doi: 10.1039/c7tb02682j. Epub 2018 Jan 8.
Oral plaque biofilms are highly resilient microbial assemblies that are challenging to eradicate. Herein, we describe the synthesis and study of pH-positive, doxycycline (DOX)-loaded nanocarriers to combat pathogenic biofilms. The mixed shell-core nanoparticles consisted of quaternary ammonium chitosan (TMC) as a positively charged section, which targeted nanoparticles to negatively charged biofilm surfaces. In addition liposomes were used as a DOX loading tool to eradicate the multidrug-resistant biofilm. In a drug release test, DOX release was pH-dependent with t = 0.75 h and 2.3 h for release at pH 4.5 and 6.8, respectively. Furthermore, TMC-Lip-DOX NPs could adhere to the biofilm and efficiently remove the biofilm from the hydroxyapatite (HA) surface. Furthermore, TMC-Lip-DOX NPs had biocomaptible properties and were non-toxic to MC3T3-E1 cells. This constitutes a highly effective pathway to control oral plaque biofilms and has a good potential use for dental biofilm therapies.
口腔菌斑生物膜是极具韧性的微生物聚集体，难以根除。在此，我们描述了用于对抗致病性生物膜的pH阳性、负载强力霉素（DOX）的纳米载体的合成与研究。混合的核壳纳米颗粒由作为带正电荷部分的季铵壳聚糖（TMC）组成，它使纳米颗粒靶向带负电荷的生物膜表面。此外，脂质体被用作DOX负载工具以根除多重耐药生物膜。在药物释放测试中，DOX释放呈pH依赖性，在pH 4.5和6.8时的释放时间分别为t = 0.75小时和2.3小时。此外，TMC-Lip-DOX纳米颗粒可附着于生物膜并有效从羟基磷灰石（HA）表面去除生物膜。此外，TMC-Lip-DOX纳米颗粒具有生物相容性，对MC3T3-E1细胞无毒。这构成了控制口腔菌斑生物膜的高效途径，在牙科生物膜治疗中具有良好的潜在应用价值。
