Xu Chunlin, Zhang Molino Binbin, Wang Xiaoju, Cheng Fang, Xu Wenyang, Molino Paul, Bacher Markus, Su Dandan, Rosenau Thomas, Willför Stefan, Wallace Gordon
Johan Gadolin Process Chemistry Centre, Åbo Akademi University, Turku, Finland.
J Mater Chem B. 2018 Nov 21;6(43):7066-7075. doi: 10.1039/c8tb01757c. Epub 2018 Oct 22.
We present for the first time approaches to 3D-printing of nanocellulose hydrogel scaffolds based on double crosslinking, first by in situ Ca crosslinking and post-printing by chemical crosslinking with 1,4-butanediol diglycidyl ether (BDDE). Scaffolds were successfully printed from 1% nanocellulose hydrogels, with their mechanical strength being tunable in the range of 3 to 8 kPa. Cell tests suggest that the 3D-printed and BDDE-crosslinked nanocellulose hydrogel scaffolds supported fibroblast cells' proliferation, which was improving with increasing rigidity. These 3D-printed scaffolds render nanocellulose a new member of the family of promising support structures for crucial cellular processes during wound healing, regeneration and tissue repair.
我们首次展示了基于双重交联的纳米纤维素水凝胶支架的3D打印方法，首先是通过原位钙交联，然后在打印后通过与1,4-丁二醇二缩水甘油醚（BDDE）进行化学交联。成功地从1%的纳米纤维素水凝胶中打印出支架，其机械强度可在3至8 kPa范围内调节。细胞测试表明，3D打印并经BDDE交联的纳米纤维素水凝胶支架支持成纤维细胞的增殖，且随着刚度的增加而改善。这些3D打印支架使纳米纤维素成为在伤口愈合、再生和组织修复过程中对关键细胞过程有前景的支持结构家族的新成员。
