Yang Yingjun, Wang Xinlong, Huang Tsung-Chun, Hu Xiaohong, Kawazoe Naoki, Tsai Wei-Bor, Yang Yingnan, Chen Guoping
Tissue Regeneration Materials Group, Research Center of Functional Materials, National Institute for Materials Science, 1-1 Namiki, Tsukuba, Ibaraki 305-0044, Japan.
J Mater Chem B. 2018 Sep 14;6(34):5424-5434. doi: 10.1039/c8tb01621f. Epub 2018 Aug 2.
Micro- and nano-structured substrates have been widely used in the biomedical engineering field. Their precise control of cell morphology makes them promising for investigating various cell behaviors. However, regulation of cell functions using micro-nano hybrid patterns is rarely achieved. Since the cell microenvironment in vivo has complex micro- and nano-structures, it is desirable to use micro-nano hybrid patterns to mimic the microenvironment to control cell morphology and disclose its influence on stem cell differentiation. In this study, poly(vinyl alcohol) (PVA) micro-stripes with different spacings (50 μm, 100 μm and 200 μm) were constructed on polystyrene (PS) nano-grooves to prepare micro-nano hybrid patterns where the direction of the PVA micro-stripes and PS nano-grooves was parallel or orthogonal. Human bone marrow-derived mesenchymal stem cells (hMSCs) cultured on the micro-nano hybrid patterns showed a different cell alignment and elongation dependent on the PVA micro-stripe spacing and orientation of the PS nano-grooves. Comparison of the influence of cell alignment and aspect ratio on differentiation of hMSCs indicated that myogenic differentiation was predominantly regulated by cell alignment and osteogenic differentiation by cell elongation, while adipogenic differentiation was regulated neither by cell alignment nor by cell elongation.
微纳结构基底已在生物医学工程领域中得到广泛应用。它们对细胞形态的精确控制使其在研究各种细胞行为方面具有广阔前景。然而，利用微纳混合图案调控细胞功能的研究却鲜有报道。由于体内细胞微环境具有复杂的微纳结构，因此使用微纳混合图案来模拟微环境以控制细胞形态并揭示其对干细胞分化的影响是很有必要的。在本研究中，在聚苯乙烯（PS）纳米凹槽上构建了具有不同间距（50μm、100μm和200μm）的聚乙烯醇（PVA）微条纹，以制备PVA微条纹与PS纳米凹槽方向平行或正交的微纳混合图案。在微纳混合图案上培养的人骨髓间充质干细胞（hMSCs）表现出不同的细胞排列和伸长情况，这取决于PVA微条纹的间距和PS纳米凹槽的方向。对细胞排列和纵横比对hMSCs分化影响的比较表明，成肌分化主要受细胞排列调控，成骨分化受细胞伸长调控，而成脂分化既不受细胞排列也不受细胞伸长的调控。
