Murray Alan F, Delivopoulos Evangelos
School of Engineering, University of Edinburgh, Edinburgh EH9 3FB, UK.
School of Biological Sciences, University of Reading, Reading RG6 6DH, UK.
Materials (Basel). 2021 Jun 9;14(12):3174. doi: 10.3390/ma14123174.
Neuronal patterning on microfabricated architectures has developed rapidly over the past few years, together with the emergence of soft biocompatible materials and tissue engineering scaffolds. Previously, we introduced a patterning technique based on serum and the biopolymer parylene-C, achieving highly compliant growth of primary neurons and astrocytes on different geometries. Here, we expanded this technique and illustrated that neuralized cells derived from mouse embryonic stem cells (mESCs) followed stripes of variable widths with conformity equal to or higher than that of primary neurons and astrocytes. Our results indicate the presence of undifferentiated mESCs, which also conformed to the underlying patterns to a high degree. This is an exciting and unexpected outcome, as molecular mechanisms governing cell and ECM protein interactions are different in stem cells and primary cells. Our study enables further investigations into the development and electrophysiology of differentiating patterned neural stem cells.
在过去几年中，随着生物相容性软材料和组织工程支架的出现，微纳加工结构上的神经元图案化技术发展迅速。此前，我们引入了一种基于血清和生物聚合物聚对二甲苯-C的图案化技术，实现了原代神经元和星形胶质细胞在不同几何形状上的高度顺应性生长。在此，我们扩展了该技术，并表明源自小鼠胚胎干细胞（mESCs）的神经化细胞沿着不同宽度的条纹生长，其顺应性等于或高于原代神经元和星形胶质细胞。我们的结果表明存在未分化的mESCs，它们也高度顺应底层图案。这是一个令人兴奋且意想不到的结果，因为干细胞和原代细胞中控制细胞与细胞外基质蛋白相互作用的分子机制不同。我们的研究有助于进一步研究分化的图案化神经干细胞的发育和电生理学。
