Research Center for Functional Materials, National Institute for Materials Science, Tsukuba, Ibaraki, Japan.
Adv Exp Med Biol. 2020;1250:141-155. doi: 10.1007/978-981-15-3262-7_10.
Micro-patterned surfaces have been broadly used to control the morphology of stem cells for investigation of the influence of physiochemical and biological cues on stem cell functions. Different structures of micro-patterned surfaces can be prepared by photolithography through designing the photomask features. Cell spreading area, geometry, aspect ratio, and alignment can be regulated by the micro-patterned structures. Their influences on adipogenic, osteogenic, and smooth muscle differentiation of the human bone marrow-derived mesenchymal stem cells are compared and investigated in details. Variation of cell morphology can trigger rearrangement of cytoskeleton, generating cytoskeletal mechanical stimulation and consequently inducing differentiation of mesenchymal stem cells into different lineages. This chapter summarizes the latest development of regulation of mesenchymal stem cell morphology by micro-patterns and the influence on the behaviors and differentiation of the mesenchymal stem cells.
微图案表面已被广泛用于控制干细胞的形态，以研究物理化学和生物线索对干细胞功能的影响。通过设计光掩模特征，可以通过光刻来制备不同结构的微图案表面。微图案结构可以调节细胞的铺展面积、几何形状、纵横比和取向。详细比较和研究了它们对人骨髓间充质干细胞成脂、成骨和成平滑肌分化的影响。细胞形态的变化会引发细胞骨架的重排，产生细胞骨架机械刺激，从而诱导间充质干细胞向不同谱系分化。本章总结了微图案对间充质干细胞形态的调控最新进展及其对间充质干细胞行为和分化的影响。
