Regulation of Stem Cell Functions by Micro-Patterned Structures.

Micro-patterned surfaces have been broadly used to control the morphology of stem cells for investigation of the influence of physiochemical and biological cues on stem cell functions. Different structures of micro-patterned surfaces can be prepared by photolithography through designing the photomask features. Cell spreading area, geometry, aspect ratio, and alignment can be regulated by the micro-patterned structures. Their influences on adipogenic, osteogenic, and smooth muscle differentiation of the human bone marrow-derived mesenchymal stem cells are compared and investigated in details. Variation of cell morphology can trigger rearrangement of cytoskeleton, generating cytoskeletal mechanical stimulation and consequently inducing differentiation of mesenchymal stem cells into different lineages. This chapter summarizes the latest development of regulation of mesenchymal stem cell morphology by micro-patterns and the influence on the behaviors and differentiation of the mesenchymal stem cells.