Su Haiyang, Liu Tingting, Huang Lin, Huang Jingyi, Cao Jing, Yang Hengquan, Ye Jian, Liu Jian, Qian Kun
School of Biomedical Engineering, Med-X Research Institute, Shanghai Jiao Tong University, Shanghai 200030, People's Republic of China.
J Mater Chem B. 2018 Nov 28;6(44):7280-7287. doi: 10.1039/c8tb01587b. Epub 2018 Aug 14.
Advanced bio-analytical platforms rely on materials with tailored structural parameters. Herein, we assembled plasmonic Janus hybrids for laser desorption/ionization mass spectrometry (LDI MS) detection of small metabolites. We applied Janus particles (periodic mesoporous organosilica (PMO) in conjunction with carbon spheres) as core materials and loaded silver nanoparticles as nanoshells. Janus core particles enhanced the LDI MS detection of both hyhrophobic and hydrophilic molecules due to anisotropic properties. Moreover, plasmonic nanoshells enabled direct metabolite detection with silver adduction in bio-mixtures, due to the selective LDI process. Furthermore, we demonstrated the tunable surface chemistry of Janus hybrids for the enrichment and identification of metabolites at low concentration (4 pM). Our work contributed to the development of tools for metabolic analysis and design of materials for bio-analytical platforms.
先进的生物分析平台依赖于具有定制结构参数的材料。在此，我们组装了用于激光解吸/电离质谱（LDI MS）检测小分子代谢物的等离子体Janus杂化物。我们将Janus颗粒（周期性介孔有机硅（PMO）与碳球结合）用作核心材料，并负载银纳米颗粒作为纳米壳。Janus核心颗粒由于其各向异性特性，增强了对疏水性和亲水性分子的LDI MS检测。此外，由于选择性LDI过程，等离子体纳米壳能够在生物混合物中通过银加合直接检测代谢物。此外，我们展示了Janus杂化物可调节的表面化学性质，用于低浓度（4 pM）代谢物的富集和鉴定。我们的工作有助于代谢分析工具的开发以及生物分析平台材料的设计。
