Wu Shu, Qian Linxi, Huang Lin, Sun Xuming, Su Haiyang, Gurav Deepanjali D, Jiang Mawei, Cai Wei, Qian Kun
School of Biomedical Engineering, Med-X Research Institute, Shanghai Jiao Tong University, Shanghai, 200030, People's Republic of China.
Xinhua Hospital, Shanghai Institute for Pediatric Research, Shanghai Jiao Tong University, Shanghai, 200092, People's Republic of China.
Nanomicro Lett. 2018;10(3):52. doi: 10.1007/s40820-018-0204-6. Epub 2018 May 17.
Nutriology relies on advanced analytical tools to study the molecular compositions of food and provide key information on sample quality/safety. Small nutrients detection is challenging due to the high diversity and broad dynamic range of molecules in food samples, and a further issue is to track low abundance toxins. Herein, we developed a novel plasmonic matrix-assisted laser desorption/ionization mass spectrometry (MALDI MS) approach to detect small nutrients and toxins in complex biological emulsion samples. Silver nanoshells (SiO@Ag) with optimized structures were used as matrices and achieved direct analysis of ~ 6 nL of human breast milk without any enrichment or separation. We performed identification and quantitation of small nutrients and toxins with limit-of-detection down to 0.4 pmol (for melamine) and reaction time shortened to minutes, which is superior to the conventional biochemical method currently in use. The developed approach contributes to the near-future application of MALDI MS in a broad field and personalized design of plasmonic materials for real-case bio-analysis.
营养科学依靠先进的分析工具来研究食物的分子组成，并提供有关样品质量/安全性的关键信息。由于食品样品中分子的高度多样性和广泛的动态范围，小分子营养素的检测具有挑战性，另一个问题是追踪低丰度毒素。在此，我们开发了一种新型的等离子体基质辅助激光解吸/电离质谱（MALDI MS）方法，用于检测复杂生物乳液样品中的小分子营养素和毒素。具有优化结构的银纳米壳（SiO@Ag）被用作基质，无需任何富集或分离即可直接分析约6 nL的人母乳。我们对小分子营养素和毒素进行了鉴定和定量，检测限低至0.4 pmol（对于三聚氰胺），反应时间缩短至几分钟，这优于目前使用的传统生化方法。所开发的方法有助于MALDI MS在不久的将来在广泛领域的应用以及用于实际生物分析的等离子体材料的个性化设计。
