Li Wenying, Wang Jiayan, Zhu Jiacheng, Zheng Yue-Qing
Research Center of Applied Solid State Chemistry, Chemistry Institute for Synthesis and Green Application, Ningbo University, Ningbo 315211, Zhejiang, P. R. China.
J Mater Chem B. 2018 Nov 14;6(42):6858-6864. doi: 10.1039/c8tb01948g. Epub 2018 Oct 12.
Nanomaterial-based artificial enzymes (nanozymes), as an emerging generation of artificial enzymes, have received extensive attention in recent years owing to their striking merits. In this study, the obtained CoO nanocrystals exhibited catalase-like activity and could catalyze the oxidation of 3,3',5,5'-tetramethylbenzidine (TMB) in the presence of HO, and a clear absorption peak at 652 nm can be observed. It was verified that the catalytic activity for the oxidation of TMB originated from the oxygen, which is generated by CoO catalyzed HO decomposition. Upon addition of glutathione (GSH), the catalytic ability of the CoO nanocrystals was inhibited. And, the changes of absorbance at 652 nm could be utilized to quantify the concentration of GSH. Under the optimized conditions, the proposed assay showed good linear relationships and a low detection limit towards GSH. Moreover, the CoO nanocrystals had excellent stability and can maintain the catalytic activity for a long time. Thus, a simple, sensitive and selective nanozyme-based biosensor was developed for the colorimetric detection of GSH.
基于纳米材料的人工酶（纳米酶）作为新一代人工酶，近年来因其显著优点而受到广泛关注。在本研究中，所制备的氧化钴纳米晶体表现出类过氧化氢酶活性，在过氧化氢存在的情况下能够催化3,3',5,5'-四甲基联苯胺（TMB）的氧化反应，并且在652 nm处可观察到明显的吸收峰。经证实，TMB氧化反应的催化活性源自氧化钴催化过氧化氢分解产生的氧气。加入谷胱甘肽（GSH）后，氧化钴纳米晶体的催化能力受到抑制。并且，652 nm处吸光度的变化可用于定量GSH的浓度。在优化条件下，所提出的检测方法对GSH表现出良好的线性关系和低检测限。此外，氧化钴纳米晶体具有出色的稳定性，能够长时间保持催化活性。因此，开发了一种用于比色检测GSH的简单、灵敏且选择性高的基于纳米酶的生物传感器。
