Karkavelas G, Kefalides N A, Amenta P S, Martinez-Hernandez A
Department of Medicine, University of Pennsylvania, Philadelphia 19104.
J Ultrastruct Mol Struct Res. 1988 Aug;100(2):137-55. doi: 10.1016/0889-1605(88)90021-3.
Antibodies against collagen types III and VI have been localized by electron immunohistochemistry with two different techniques in normal rat uterus and kidney. Antibodies directed against two components of the extracellular matrix with known localization, laminin and type IV collagen, were used as controls for the specificity of the localization. The results demonstrate that types III and VI are found in the interstitium as fine (10- to 15-nm), beaded fibrils and filaments (6- to 10-nm), respectively. Both are often found associated with thick, crossbanded type I collagen fibers (30- to 35-nm) and occasionally associated with some basement membranes adjacent to the interstitium. Further, the findings suggest that collagens III and VI may connect the various components of the extracellular matrix, such as type I fibers with basement membranes and other structures, thus forming an integrated functional unit.
利用两种不同技术，通过电子免疫组织化学方法，在正常大鼠子宫和肾脏中对III型和VI型胶原抗体进行了定位。针对细胞外基质中已知定位的两种成分（层粘连蛋白和IV型胶原）的抗体，用作定位特异性的对照。结果表明，III型和VI型分别以纤细（10至15纳米）、串珠状纤维和细丝（6至10纳米）的形式存在于间质中。二者常与粗大的、有横纹的I型胶原纤维（30至35纳米）相关联，偶尔也与间质相邻的一些基底膜相关联。此外，研究结果表明，III型和VI型胶原可能连接细胞外基质的各种成分，如I型纤维与基底膜及其他结构，从而形成一个整合功能单元。
