Vallejos Saúl, Hernando Elsa, Trigo Miriam, García Félix C, García-Valverde María, Iturbe David, Cabero María Jesús, Quesada Roberto, García José M
Departamento de Química, Facultad de Ciencias, Universidad de Burgos, Plaza de Misael Bañuelos s/n, 09001 Burgos, Spain.
J Mater Chem B. 2018 Jun 14;6(22):3735-3741. doi: 10.1039/c8tb00682b. Epub 2018 May 23.
We have developed a new extremely hydrophilic polymeric film suitable for the detection and quantification of chloride in human sweat directly on the skin. The film, or membrane, has chemically anchored 6-methoxyquinoline groups as chloride responsive fluorescent motifs. We have prepared the sensory material from a standard vinyl copolymer, by a convenient and easy solid-phase reaction. The sensory material has a water swelling percentage of 700%, facilitating an immediate detection of chloride, is reusable for at least 6 cycles and can be handled without care by unskilled persons. The initially high fluorescence of the material decreases in the presence of chloride, allowing the quantification of chloride concentration by using the colour definition of a digital picture or a fluorimeter. The suitability of the material to perform quantitative chloride analysis of human sweat by putting it in contact with the skin offers promise for its application in the sweat test used for the diagnosis of cystic fibrosis (CF).
我们开发了一种新型的超亲水性聚合物薄膜，适用于直接在皮肤上检测和定量人体汗液中的氯化物。该薄膜或膜片化学固定了6-甲氧基喹啉基团作为对氯化物有响应的荧光基序。我们通过一种简便的固相反应，由标准乙烯基共聚物制备了这种传感材料。该传感材料的水溶胀率为700%，便于即时检测氯化物，可重复使用至少6个循环，并且非专业人员也能轻松操作。在氯化物存在的情况下，该材料最初的高荧光会降低，通过使用数码照片的颜色定义或荧光计可以对氯化物浓度进行定量。将该材料与皮肤接触进行人体汗液中氯化物定量分析的适用性，为其在用于诊断囊性纤维化（CF）的汗液测试中的应用带来了希望。
