Department of Functional Materials in Medicine and Dentistry and Bavarian Polymer Institute (BPI), University of Würzburg, Pleicherwall 2, 97070 Würzburg, Germany.
J Mater Chem B. 2019 Mar 14;7(10):1782-1794. doi: 10.1039/c8tb02575d. Epub 2019 Feb 13.
We describe the preparation of hydrogels using highly functionalized poly(oxazoline) based polymeric precursors and cross-linking via UV mediated radical thiol-ene chemistry. Random copolymers were synthesized based on the combination of the more hydrophilic 2-methyl-2-oxazoline or the less hydrophilic monomer 2-ethyl-2-oxazoline with 2-(3-butenyl)-2-oxazoline. These copolymers were functionalized via a post-polymerization technique with thiol or cysteine functionality at the side chain. Hence, hydrogels were obtained, for which the thermo-responsive behavior, network density and correlated properties such as swelling and mechanics, as well as the possibility of electrostatic interaction, can be tuned. Cell culture tests demonstrated good cytocompatibility of the synthesized copolymers and hydrogels. A study with two low molecular weight substances, methylene blue and fluorescein sodium, was performed to investigate how the thermo-responsive behavior or the positive charge incorporated by cysteine could influence the interaction with the compounds. It was found that the interaction with the hydrogel network was strongly influenced by the chemical properties of the dye. A hydrophilic and positively charged hydrogel network was shown to be a promising candidate for the uptake and prolonged release of negatively charged low molecular weight substances.
我们描述了使用高度官能化的聚恶唑啉基聚合物前体通过 UV 介导的自由基硫醇-烯反应交联制备水凝胶。基于更亲水的 2-甲基-2-恶唑啉或疏水性较小的单体 2-乙基-2-恶唑啉与 2-(3-丁烯基)-2-恶唑啉的组合合成了无规共聚物。这些共聚物通过后聚合技术用侧链上的巯基或半胱氨酸官能团进行功能化。因此，获得了水凝胶，其热响应行为、网络密度和相关性质（如溶胀和力学性能）以及静电相互作用的可能性可以进行调节。细胞培养测试表明合成共聚物和水凝胶具有良好的细胞相容性。对两种低分子量物质亚甲蓝和荧光素钠进行了研究，以研究半胱氨酸引入的热响应行为或正电荷如何影响与化合物的相互作用。结果发现，与水凝胶网络的相互作用强烈受到染料化学性质的影响。具有亲水性和正电荷的水凝胶网络有望成为吸收和延长释放带负电荷的低分子量物质的有前途的候选物。
