Zhang Weijie, Huo Fangjun, Liu Tao, Yin Caixia
Key Laboratory of Chemical Biology and Molecular Engineering of Ministry of Education, Key Laboratory of Materials for Energy, Conversion and Storage of Shanxi Province, Institute of Molecular Science, Shanxi University, Taiyuan 030006, China.
J Mater Chem B. 2018 Dec 28;6(48):8085-8089. doi: 10.1039/c8tb02536c. Epub 2018 Nov 12.
Hydrazine (NH) has been widely applied in most of the chemical industry; however, it is now believed that it can cause serious damage to protein and nucleic acid upon ingestion. Herein we introduced a specially designed molecular probe 1, with an electron deficient lactone group as a specially designed reactive site and a naphthalimide fluorophore that is easily modified owing to lactone involvement. The free probe 1 exhibited blue fluorescence in neutral buffer owing to the low favorability of ICT (intramolecular charge transfer), while the specially designed lactone group could potentially undergo a ring-opening reaction with hydrazine, resulting in a favoured ICT effect and bathochromically shifted (125 nm) fluorescence response. The ratiometric fluorescence signal enabled quantitative determination of hydrazine using probe 1 in complex applications. Besides, probe 1 features a rapid signal response time (within 5 min) and low detection limit (36 nM) for hydrazine. Utilizing these excellent properties, probe 1 was further applied in hydrazine vapor detection and bio-imaging. Conceptually, we now present a new approach for the detection of hydrazine, and the enhanced performances of probe 1 will promote its applications in living systems and the environment.
肼（NH）已在大多数化学工业中广泛应用；然而，现在人们认为，摄入肼会对蛋白质和核酸造成严重损害。在此，我们介绍了一种特别设计的分子探针1，它具有缺电子内酯基团作为特殊设计的反应位点，以及由于内酯参与而易于修饰的萘二甲酰亚胺荧光团。游离探针1在中性缓冲液中由于分子内电荷转移（ICT）的低倾向性而呈现蓝色荧光，而特别设计的内酯基团可能会与肼发生开环反应，从而产生有利的ICT效应和红移（125 nm）荧光响应。比率荧光信号能够在复杂应用中使用探针1对肼进行定量测定。此外，探针1对肼具有快速的信号响应时间（5分钟内）和低检测限（36 nM）。利用这些优异性能，探针1进一步应用于肼蒸气检测和生物成像。从概念上讲，我们现在提出了一种检测肼的新方法，并且探针1的增强性能将促进其在生命系统和环境中的应用。
