一种基于信息通信技术的用于检测肼的香豆素荧光探针及其在环境水样和生物体中的应用。
An ICT-Based Coumarin Fluorescent Probe for the Detection of Hydrazine and Its Application in Environmental Water Samples and Organisms.
作者信息
Liu Xina, Zhu Meiqing, Xu Chenyang, Fan Fugang, Chen Panpan, Wang Yi, Li Dongyang
机构信息
Anhui Provincial Key Laboratory of Quality and Safety of Agricultural Products, College of Resources and Environment, Anhui Agricultural University, Hefei, China.
School of Chemical and Environmental Engineering, Anhui Polytechnic University, Wuhu, China.
出版信息
Front Bioeng Biotechnol. 2022 Jun 14;10:937489. doi: 10.3389/fbioe.2022.937489. eCollection 2022.
As an inorganic small molecule pollutant, the toxicity and potential carcinogenicity of hydrazine (NH) are of increasing concern. In this work, A water-soluble fluorescent probe (OCYB) based on the intramolecular charge transfer (ICT) mechanism for the detection of hydrazine was designed and synthesized. Taking the advantage of 4-bromobutyryl as the recognition group, the high selectivity of OCYB to NH was confirmed by steady-state fluorescence spectroscopy. The limit of detection (LOD) was calculated to be 78 nM in the DMSO-HEPES (pH 7.4) system. The detection mechanism was verified by NMR, HRMS and density functional theory (DFT) calculations. In addition, OCYB exhibits strong anti-interference ability and an "Off-On" fluorescence enhancement effect. Importantly, OCYB can be used to effectively monitor the fluorescence distribution of NH in environmental water samples and organisms.
作为一种无机小分子污染物，肼（NH）的毒性和潜在致癌性日益受到关注。在本工作中，设计并合成了一种基于分子内电荷转移（ICT）机制用于检测肼的水溶性荧光探针（OCYB）。利用4-溴丁酰基作为识别基团，通过稳态荧光光谱证实了OCYB对NH的高选择性。在DMSO-HEPES（pH 7.4）体系中，检测限（LOD）计算为78 nM。通过核磁共振（NMR）、高分辨质谱（HRMS）和密度泛函理论（DFT）计算验证了检测机理。此外，OCYB表现出较强的抗干扰能力和“关-开”荧光增强效应。重要的是，OCYB可用于有效监测环境水样和生物体中NH的荧光分布。
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