Zhou Changchun, Jiang Yi, Sun Zhihui, Li Yanyan, Guo Bo, Hong Youliang
National Engineering Research Centre for Biomaterials, Sichuan University, Chengdu, 610064, P. R. China.
J Mater Chem B. 2018 Sep 21;6(35):5621-5632. doi: 10.1039/c8tb01638k. Epub 2018 Aug 23.
Although calcium phosphate (CaP) ceramics have been originally defined as bioactive materials because a biologically active hydroxycarbonate apatite (HCA) layer can form on their surfaces, the biological effects of the as-grown HCA layers are far from understood. In particular, it is unclear whether the as-grown HCA nanotopography can mediate the osteogenic commitment of mesenchymal stem cells (MSCs). In this study, a systematic investigation was performed to investigate the formation and biological effects of HCA nanotopography on CaP ceramic surfaces. Experiments demonstrate that the hydroxyapatite phase-containing CaP ceramics tend to grow HCA nanoparticle-constructed nanotopography, which can mediate bone marrow MSCs to condensate and spontaneously differentiate toward osteogenic lineage. In addition, the biological evolution of MSCs adhered on such nanotopography is similar to intramembranous ossification. Our findings provide support for applications of wurtzite phase-containing CaP ceramics in regenerative medicine for hard tissues.
尽管磷酸钙（CaP）陶瓷最初被定义为生物活性材料，因为在其表面可以形成具有生物活性的羟基碳酸盐磷灰石（HCA）层，但对于生长形成的HCA层的生物学效应却知之甚少。特别是，目前尚不清楚生长形成的HCA纳米拓扑结构是否能够介导间充质干细胞（MSC）的成骨定向分化。在本研究中，我们进行了系统的研究，以探究CaP陶瓷表面HCA纳米拓扑结构的形成及其生物学效应。实验表明，含羟基磷灰石相的CaP陶瓷倾向于生长由HCA纳米颗粒构建的纳米拓扑结构，这种结构能够介导骨髓间充质干细胞凝聚并自发地向成骨谱系分化。此外，附着在这种纳米拓扑结构上的间充质干细胞的生物学演变过程类似于膜内成骨。我们的研究结果为含纤锌矿相的CaP陶瓷在硬组织再生医学中的应用提供了支持。
