Bi Xuexin, Su Huiling, Shi Wei, Liu Xin, He Zi, Zhang Xiuming, Sun Yanan, Ge Dongtao
Key Laboratory of Biomedical Engineering of Fujian Province University/Research Center of Biomedical Engineering of Xiamen, Department of Biomaterials, College of Materials, Xiamen University, Xiamen 361005, China.
J Mater Chem B. 2018 Dec 21;6(47):7877-7888. doi: 10.1039/c8tb01921e. Epub 2018 Nov 14.
A facile reverse microemulsion method was developed for the synthesis of homogenous poly(pyrrole-3-carboxylic acid) (PPyCOOH) nanoparticles, which used n-heptane as the oil phase, sodium dodecyl sulfate as the surfactant, 1-butanol as the co-surfactant and pyrrole-3-carboxylic solution as the aqueous phase. The obtained PPyCOOH nanoparticles had uniform sizes, high near-infrared absorption and abundant functional groups. To improve their stability and enhance the tumor inhibition rate, the PPyCOOH nanoparticles were modified with bovine serum albumin (BSA) and doxorubicin (DOX). The as-fabricated drug delivery system (DOX@BSA-PPyCOOH) combining photothermal therapy and chemotherapy exhibited pH-sensitive and near infrared laser controlled drug release, which was beneficial for drug enrichment in the tumor and for reducing the toxicity and side effects in normal tissue. Combined chemo-photothermal therapy in vivo revealed that the tumors were completely eliminated and no obvious toxicity was found in the major organs, suggesting that DOX@BSA-PPyCOOH nanoparticles are an efficient nanoagent for cancer treatment.
开发了一种简便的反相微乳液法来合成均匀的聚（吡咯 - 3 - 羧酸）（PPyCOOH）纳米颗粒，该方法以正庚烷为油相，十二烷基硫酸钠为表面活性剂，1 - 丁醇为助表面活性剂，吡咯 - 3 - 羧酸溶液为水相。所制备的PPyCOOH纳米颗粒尺寸均匀，具有高近红外吸收和丰富的官能团。为了提高其稳定性并提高肿瘤抑制率，用牛血清白蛋白（BSA）和阿霉素（DOX）对PPyCOOH纳米颗粒进行了修饰。所制备的结合光热疗法和化疗的药物递送系统（DOX@BSA - PPyCOOH）表现出pH敏感和近红外激光控制的药物释放，这有利于药物在肿瘤中的富集并降低正常组织中的毒性和副作用。体内联合化学 - 光热疗法表明肿瘤被完全消除，主要器官未发现明显毒性，这表明DOX@BSA - PPyCOOH纳米颗粒是一种有效的癌症治疗纳米制剂。
