Institute of Burn Research, Southwest Hospital, State Key Lab of Trauma, Burn and Combined Injury, Chongqing Key Laboratory for Disease Proteomics, Third Military Medical University (Army Medical University), Chongqing, 400038, China.
Institute of Burn Research, Southwest Hospital, State Key Lab of Trauma, Burn and Combined Injury, Chongqing Key Laboratory for Disease Proteomics, Third Military Medical University (Army Medical University), Chongqing, 400038, China; Department of Pharmaceutics, College of Pharmacy, Third Military Medical University (Army Medical University), Chongqing, 400038, China.
Redox Biol. 2022 Aug;54:102372. doi: 10.1016/j.redox.2022.102372. Epub 2022 Jun 17.
Thionitrous acid (HSNO), a crosstalk intermediate of two crucial gasotransmitters nitric oxide and hydrogen sulfide, plays a critical role in redox regulation of cellular signaling and functions. However, real-time and facile detection of HSNO with high selectivity and sensitivity remains highly challenging. Herein we report a novel fluorescent probe (SNP-1) for HSNO detection. SNP-1 has a simple molecular structure, but showing strong fluorescence, a low detection limit, a broad linear detection range (from nanomolar to micromolar concentrations), ultrasensitivity, and high selectivity for HSNO in both aqueous media and cells. Benefiting from these unique features, SNP-1 could effectively visualize changes of HSNO levels in mouse models of acute ulcerative colitis and renal ischemia/reperfusion injury. Moreover, the good correlation between colonic HSNO levels and disease activity index demonstrated that HSNO is a promising new diagnostic agent for acute ulcerative colitis. Therefore, SNP-1 can serve as a useful fluorescent probe for precision detection of HSNO in various biological systems, thereby facilitating mechanistic studies, therapeutic assessment, and high-content drug screening for corresponding diseases.
硫亚硝酰（HSNO）是两种关键气体递质一氧化氮和硫化氢的串扰中间体，在细胞信号转导和功能的氧化还原调节中起着关键作用。然而，具有高选择性和灵敏度的 HSNO 的实时和简便检测仍然极具挑战性。在此，我们报告了一种用于 HSNO 检测的新型荧光探针（SNP-1）。SNP-1 具有简单的分子结构，但显示出强荧光、低检测限、宽线性检测范围（从纳摩尔到微摩尔浓度）、超高灵敏度和对水相介质和细胞中 HSNO 的高选择性。得益于这些独特的特性，SNP-1 可以有效地可视化急性溃疡性结肠炎和肾缺血/再灌注损伤小鼠模型中 HSNO 水平的变化。此外，结肠 HSNO 水平与疾病活动指数之间的良好相关性表明，HSNO 是急性溃疡性结肠炎有前途的新型诊断试剂。因此，SNP-1 可以作为一种有用的荧光探针，用于各种生物系统中 HSNO 的精确检测，从而促进相应疾病的机制研究、治疗评估和高内涵药物筛选。
