Clin Lab. 2020 Apr 1;66(4). doi: 10.7754/Clin.Lab.2019.190734.
microRNAs (miRNAs) have an important role in cancer development and progression. It has been shown that miR-372 and miR-101 are involved in cancer progression. In the present study we evaluated expressions of these miRNAs and their serum levels in patients with head and neck squamous cell carcinoma (HNSCC) and controls.
We conducted this case-control study on 60 patients with HNSCC and 30 controls. Patients were diagnosed by histological assessments of their tissues. Expressions of EGFR, PTEN, PI3K/CA, miR-372, and miR-101a were evaluated in the tissues, along with serum levels of the miRNAs.
Tissue expression of PTEN decreased in HNSCC, and expressions of EGFR and PI3K increased in HNSCC tissues compared to the controls. Tissue expressions of miR-372 increased and miR-101a decreased in HNSCC tissues compared to the controls. We observed significantly lower serum levels of miR-101a in patients; however, these findings for miR-372 were not significant. A strong correlation existed between serum levels and tissue expression of miR-101a. Notably, miR-101a serum levels showed good sensitivity and specificity for diagnosis of HNSCC.
The results showed that HNSCC patients had higher tissue expression of miR-372 and lower expression of miR-101a. Also, serum levels of miR-101a were lower in HNSCC patients. We observed that miR-101a had good sensitivity and specificity for diagnosis of HNSCC. The present study suggested that miR-101a could be a potential biomarker for HNSCC.
微小 RNA（miRNA）在癌症的发生和发展中起着重要作用。已经证明 miR-372 和 miR-101 参与了癌症的进展。在本研究中，我们评估了这些 miRNA 在头颈部鳞状细胞癌（HNSCC）患者和对照组中的表达及其血清水平。
我们对 60 例 HNSCC 患者和 30 例对照组进行了病例对照研究。通过对组织的组织学评估诊断患者。评估了 EGFR、PTEN、PI3K/CA、miR-372 和 miR-101a 在组织中的表达，以及这些 miRNA 的血清水平。
与对照组相比，HNSCC 组织中 PTEN 的表达减少，EGFR 和 PI3K 的表达增加。与对照组相比，HNSCC 组织中 miR-372 的表达增加，miR-101a 的表达减少。我们观察到患者的血清 miR-101a 水平显著降低，但 miR-372 的结果并不显著。血清水平和组织表达 miR-101a 之间存在很强的相关性。值得注意的是，miR-101a 的血清水平对 HNSCC 的诊断具有良好的敏感性和特异性。
结果表明，HNSCC 患者的 miR-372 组织表达较高，miR-101a 表达较低。此外，HNSCC 患者的血清 miR-101a 水平较低。我们观察到 miR-101a 对 HNSCC 的诊断具有良好的敏感性和特异性。本研究表明，miR-101a 可能是 HNSCC 的潜在生物标志物。
