基于 X 射线介导的自由基三氟甲基化的快速蛋白质足迹分析。
Fast Protein Footprinting by X-ray Mediated Radical Trifluoromethylation.
机构信息
Department of Chemistry, Washington University, St. Louis, Missouri 63130, United States.
Center for Proteomics & Bioinformatics, School of Medicine, Case Western Reserve University, Cleveland, Ohio 44106, United States.
出版信息
J Am Soc Mass Spectrom. 2020 May 6;31(5):1019-1024. doi: 10.1021/jasms.0c00085. Epub 2020 Apr 21.
Abstract
Synchrotron radiolysis generates hydroxyl radicals (OH) that are successful footprinting reagents. Here, we describe a new reagent for the synchrotron platform, the trifluoromethyl radical (CF). The radical is produced by OH displacement of CF from sodium triflinate (Langlois reagent). Upon X-ray beam exposure, the reagent labels proteins extensively without any additional chemicals on a millisecond or shorter time scale. The CF is comparably reactive to OH and produces footprinting information that complements that of OH alone. This reagent in combination with OH should enable novel chemistry for protein footprinting on the synchrotron platform.
摘要
同步辐射光解产生羟基自由基（OH），是一种成功的足迹试剂。在这里，我们描述了一种新的同步辐射平台试剂，三氟甲基自由基（CF）。该自由基是由 OH 从三氟甲磺酸纳（朗格洛伊斯试剂）中取代 CF 产生的。在 X 射线束照射下，该试剂在毫秒或更短的时间尺度内无需任何额外的化学物质即可广泛标记蛋白质。CF 的反应性与 OH 相当，产生的足迹信息补充了单独使用 OH 的信息。这种试剂与 OH 结合使用，应该能够在同步辐射平台上实现蛋白质足迹分析的新化学方法。
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