一种商用猪繁殖与呼吸综合征病毒（PRRSV）口腔液体抗体酶联免疫吸附测定（ELISA）的现场应用。
Field application of a commercial porcine reproductive and respiratory syndrome virus (PRRSV) oral fluid antibody enzyme-linked immunosorbent assay (ELISA).
作者信息
Croft Emily, Blackwell Tim, Zimmerman Jeff
机构信息
Department of Animal Biosciences, University of Guelph, Guelph, Ontario (Croft); Ontario Ministry of Agriculture, Food, and Rural Affairs, 6484 Wellington Road 7, Unit 10, Elora, Ontario N0B 1S0 (Blackwell); Department of Veterinary Diagnostic and Production Animal Medicine, College of Veterinary Medicine, Iowa State University, Ames, Iowa 50011, USA (Zimmerman).
出版信息
Can Vet J. 2020 Apr;61(4):420-423.
A commercial porcine reproductive and respiratory syndrome virus (PRRSV) oral fluid antibody enzyme-linked immunosorbent assay (ELISA) was used on 31 commercial swine farms in Ontario using oral fluid samples (~6 per herd) collected from cotton ropes. Using the manufacturer's cutoff [sample-to-positive ratio (S/P) ≥ 0.4], 2 of 135 oral fluid samples from 23 PRRSV presumed negative herds tested positive (1.5% false positive rate). Three approaches to improving test diagnostic specificity were compared: i) use a cutoff of S/P ≥ 0.8 for individual oral fluid samples; ii) use the current cutoff of S/P ≥ 0.4 but use a mean S/P based on several oral fluid samples (6 samples were used in this study); and iii) use serial testing to resolve unexpected positive ELISA results, i.e., retest using a reverse transcription-polymerase chain reaction (RT-PCR) to determine whether low positive S/P ratios are the result of early PRRSV infection in a barn.
在安大略省的31个商业养猪场中，使用一种商业化的猪繁殖与呼吸综合征病毒（PRRSV）口腔液体抗体酶联免疫吸附测定（ELISA），检测从棉绳采集的口腔液体样本（每个猪群约6份）。按照制造商的临界值[样本与阳性比值（S/P）≥0.4]，来自23个推测为PRRSV阴性猪群的135份口腔液体样本中有2份检测呈阳性（假阳性率为1.5%）。比较了三种提高检测诊断特异性的方法：i）对单个口腔液体样本使用S/P≥0.8的临界值；ii）使用当前S/P≥0.4的临界值，但基于多个口腔液体样本（本研究中使用6个样本）计算平均S/P；iii）采用系列检测来解决ELISA意外阳性结果，即使用逆转录-聚合酶链反应（RT-PCR）重新检测，以确定低阳性S/P比值是否是猪舍中PRRSV早期感染的结果。
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