Hu Xiaojing, Chang Licao, Wang Zhenyu, Liu Guosheng, Hu Zongjie, Li Ning
1Henan Engineering Laboratory for Bioconversion Technology of Functional Microbial, College of Life Sciences, Henan Normal University, Xinxiang, 453002 Henan China.
Henan Bangni Bioengineering Co. Ltd, Xinxiang, 453000 Henan China.
Indian J Microbiol. 2020 Jun;60(2):222-229. doi: 10.1007/s12088-020-00855-w. Epub 2020 Jan 8.
Sweat secretion keeps feet and insoles humid and rich in nutrients, which are the conditions needed to maintain abundant microbial growth. Analyzing the diversity and function of microorganisms in the insole is of great significance in the development of functional insoles and prediction of human foot hygiene condition. In this study, pure culture method, MiSeq high-throughput sequencing technology, and PICRUSt gene function prediction were used to analyze the diversity and function of the bacterial community from insoles of healthy population of different sexes and age groups. , , and are present in all insole samples, and there is no significant difference between sexes of the same age group. However, a significant difference in insole microbial population was obtained among age groups. For community function, all six samples expressed similarity in the preliminary metabolism, but in samples from the elderly, many specific catabolic genes were associated with human disease and drug resistance. This study provides a reference for the development of multi-function insoles and other sanitary products for disease prediction in a healthy population.
汗液分泌使脚部和鞋垫保持湿润且营养丰富，这是维持微生物大量生长所需的条件。分析鞋垫中微生物的多样性和功能对于功能性鞋垫的开发以及人体足部卫生状况的预测具有重要意义。在本研究中，采用纯培养方法、MiSeq高通量测序技术和PICRUSt基因功能预测来分析不同性别和年龄组健康人群鞋垫中细菌群落的多样性和功能。在所有鞋垫样本中均存在[具体细菌名称1]、[具体细菌名称2]和[具体细菌名称3]，且同一年龄组的不同性别之间无显著差异。然而，不同年龄组的鞋垫微生物种群存在显著差异。对于群落功能，所有六个样本在初级代谢方面表现出相似性，但在老年样本中，许多特定的分解代谢基因与人类疾病和耐药性相关。本研究为开发多功能鞋垫及其他用于健康人群疾病预测的卫生产品提供了参考。
