Wang Guorui, Yuan Zhen, Zhang Pengyu, Liu Zhixue, Wang Tongchao, Wei Li
Agricultural College of Henan Agricultural University, Zhengzhou, 450046 Henan China.
Physiol Mol Biol Plants. 2020 Apr;26(4):705-717. doi: 10.1007/s12298-020-00770-w. Epub 2020 Feb 27.
The plant-specific NAC transcription factor (TFs) plays crucial role in plant growth as well as in stress resistance. In the present study, 87 NAC TFs were obtained from the transcriptome analysis using drought-resistant maize inbred line Y882 as experimental material under PEG stress and rewatering treatment. Comprehensive analyses were conducted including genes structure, chromosomal localization, phylogenetic tree and motif prediction, -elements and expression patterns. The results showed that the 87 genes distributed on 10 chromosomes and were categorized into 15 groups based on their conserved gene structure and motifs. Phylogenetic tree analysis was also constructed referencing to the counterparts of and rice, and the stress-related -elements in the promoter region were also analyzed. 87 genes exhibited different expression levels at 3 treatment points, indicating different response to drought stress. This genome-wide analysis of 87 genes will provide basis for further gene function detection.
植物特有的NAC转录因子在植物生长以及抗逆性方面发挥着关键作用。在本研究中，以抗旱玉米自交系Y882为实验材料，在聚乙二醇（PEG）胁迫和复水处理下进行转录组分析，获得了87个NAC转录因子。进行了包括基因结构、染色体定位、系统发育树和基序预测、顺式作用元件和表达模式在内的综合分析。结果表明，这87个基因分布在10条染色体上，根据其保守的基因结构和基序被分为15组。还参照拟南芥和水稻的对应物构建了系统发育树，并分析了启动子区域中与胁迫相关的顺式作用元件。87个基因在3个处理时间点表现出不同的表达水平，表明对干旱胁迫有不同的响应。对这87个基因的全基因组分析将为进一步的基因功能检测提供依据。
