Mishra Rukmini, Mohapatra Rupesh, Mahanty Bijayalaxmi, Joshi Raj Kumar
School of Applied Sciences, Centurion University of Technology and Management, Odisha, India.
Centre for Biotechnology, Siksha O Anusandhan University, Bhubaneswar-751030, Odisha, India.
Bioinformation. 2019 Dec 31;15(12):907-917. doi: 10.6026/97320630015907. eCollection 2019.
MicroRNAs are small non-coding RNAs of 21-24 nucleotides in length that acts as important modulators of gene expression related to numerous biological processes including development and defense response in eukaryotes. However, only a limited report on onion (Allium cepa) miRNAs is available and their associated role in growth and development of onion is not yet clear. Therefore, it is of interest to identify miRNAs and their targets in Allium cepa using the genome survey sequences (GSSs) and expressed sequence tags (ESTs) and deduce the functions of the target genes using gene ontology (GO) terms. We report 14 potential miRNAs belonging to 13 different families (miR162, miR168, miR172c, miR172e, miR398, miR400, miR414, miR1134, miR1223, miR6219, miR7725, miR8570, miR8703 and miR8752). BLAST analysis using psRNATarget server predicted 39 potential targets for the identified miRNAs majority of which were transcription factors implicated in plant growth, development, hormone signaling and stress responses. These data forms the basis for further analysis and verification towards understanding the miRNA mediated regulatory mechanism in Allium cepa.
微小RNA是长度为21 - 24个核苷酸的小型非编码RNA，在真核生物中作为与众多生物过程相关的基因表达的重要调节因子，这些生物过程包括发育和防御反应。然而，关于洋葱（葱属植物）微小RNA的报道有限，它们在洋葱生长发育中的相关作用尚不清楚。因此，利用基因组调查序列（GSS）和表达序列标签（EST）来鉴定葱属植物中的微小RNA及其靶标，并使用基因本体（GO）术语推断靶标基因的功能，是很有意义的。我们报告了属于13个不同家族的14种潜在微小RNA（miR162、miR168、miR172c、miR172e、miR398、miR400、miR414、miR1134、miR1223、miR6219、miR7725、miR8570、miR8703和miR8752）。使用psRNATarget服务器进行的BLAST分析预测了所鉴定微小RNA的39个潜在靶标，其中大多数是参与植物生长、发育、激素信号传导和应激反应的转录因子。这些数据为进一步分析和验证以了解葱属植物中微小RNA介导的调控机制奠定了基础。
