Shanghai Key Laboratory of Reproductive Medicine, Department of Histoembryology, Genetics and Developmental Biology, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, People's Republic of China.
Shanghai Key Laboratory of Orthopedic Implants, Department of Orthopedics, Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200011, People's Republic of China.
Int J Nanomedicine. 2020 Mar 17;15:1853-1862. doi: 10.2147/IJN.S237254. eCollection 2020.
Curcumin has shown many pharmacological activities in both preclinical and clinical studies. Many technologies have been developed and applied to improve the solubility and bioavailability of curcumin, especially the nanotechnology-based delivery systems. However, there has been evidence that certain nanoparticles have potential reproductive toxicity in practice.
Curcumin-poly (lactic-co-glycolic acid) (PLGA)-PEG nanoparticles (Cur-PLGA-NPs for short) were prepared. The Cur-PLGA-NPs were evaluated with its effect on the proliferation of mouse testicular cell lines in vitro and spermatogenesis in vivo, while PLGA-NPs were used as control. For animal experiments, male BALB/c mice were treated with 20 mg/kg of Cur-PLGA-NPs for continuous 10 days via tail vein injection.
We found the curcumin nanoparticles suppressed the proliferation of testicular cell lines in vitro. Furthermore, a short-term intravenous delivery of curcumin-loaded nanoparticles could be harmful to the differentiation of spermatogonia, the elongation of spermatids, as well as the motility of mature sperms.
In the present study, we disclosed the acute damage on mouse spermatogenesis and sperm parameters by curcumin-loaded nanoparticles. Our results suggested that the reproductive toxicity of nanoformulated curcumin needs to be prudently evaluated before its application.
姜黄素在临床前和临床研究中表现出许多药理学活性。许多技术已经被开发并应用于提高姜黄素的溶解度和生物利用度，特别是基于纳米技术的递药系统。然而，有证据表明某些纳米颗粒在实际中具有潜在的生殖毒性。
制备姜黄素-聚（乳酸-共-乙醇酸）（PLGA）-聚乙二醇纳米粒（简称 Cur-PLGA-NPs）。通过体外研究姜黄素-PLGA-NPs 对小鼠睾丸细胞系增殖的影响以及体内对生精作用的影响，将其与 PLGA-NPs 进行对比。对于动物实验，雄性 BALB/c 小鼠通过尾静脉注射连续 10 天给予 20mg/kg 的 Cur-PLGA-NPs。
我们发现姜黄素纳米粒抑制了体外睾丸细胞系的增殖。此外，短期静脉内给予载姜黄素纳米粒可能对精原细胞的分化、精子细胞的伸长以及成熟精子的运动产生有害影响。
在本研究中，我们揭示了载姜黄素纳米粒对小鼠生精作用和精子参数的急性损伤。我们的结果表明，在应用纳米姜黄素之前，需要谨慎评估其生殖毒性。
