State Key Laboratory of Medicinal Chemical Biology, The Key Laboratory of Bioactive Materials, The College of Life Science, Nankai University, Tianjin, People's Republic of China.
Department of Spine Surgery, Tianjin Hospital, Tianjin, People's Republic of China.
Int J Nanomedicine. 2020 Mar 20;15:1939-1950. doi: 10.2147/IJN.S235609. eCollection 2020.
Chronic trauma repair is an important issue affecting people's healthy lives. Thermo-sensitive hydrogel is injectable in situ and can be used to treat large-area wounds. In addition, antioxidants play important roles in promoting wound repair.
The purpose of this research was to prepare a novel thermo-sensitive hydrogel-poly(N-isopropyl-acrylamide)/poly(γ-glutamic acid) (PP) loaded with superoxide dismutase (SOD) to improve the effect for trauma treatment. The micromorphology of the hydrogel was observed by scanning electron microscope and the physical properties were measured. The biocompatibility of hydrogel was evaluated by MTT experiment, and the effect of hydrogel on skin wound healing was evaluated by in vivo histological staining.
Gelling behavior and differential scanning calorimeter outcomes showed that the PP hydrogels possessed thermo-sensitivity at physiological temperature and the phase transformation temperature was 28.2°C. The high swelling rate and good water retention were conducive to wound healing. The activity of SOD in vitro was up to 85% at 10 h, which was advantageous to eliminate the superoxide anion. MTT assay revealed that this hydrogel possessed good biocompatibility. Dressings of PP loaded with SOD (SOD-PP) had a higher wound closure rate than other treatments in vivo in diabetic rat model.
The SOD-PP thermo-sensitive hydrogels can effectively promote wound healing and have good application prospects for wound repair.
慢性创伤修复是影响人们健康生活的重要问题。温敏水凝胶可注射原位使用，可用于治疗大面积伤口。此外，抗氧化剂在促进伤口修复方面发挥着重要作用。
本研究旨在制备一种新型温敏水凝胶-聚（N-异丙基丙烯酰胺）/聚（γ-谷氨酸）（PP）负载超氧化物歧化酶（SOD），以提高创伤治疗效果。扫描电子显微镜观察水凝胶的微观形态，测量其物理性能。通过 MTT 实验评价水凝胶的生物相容性，通过体内组织学染色评价水凝胶对皮肤伤口愈合的影响。
凝胶行为和差示扫描量热法结果表明，PP 水凝胶在生理温度下具有温敏性，相转变温度为 28.2°C。高溶胀率和好的保水性能有利于伤口愈合。体外 SOD 活性在 10 h 时可达 85%，有利于消除超氧阴离子。MTT 试验表明，该水凝胶具有良好的生物相容性。负载 SOD 的 PP 敷料（SOD-PP）在糖尿病大鼠模型中的伤口闭合率高于其他治疗方法。
SOD-PP 温敏水凝胶可有效促进伤口愈合，在伤口修复方面具有良好的应用前景。
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