Research Center for Human Tissue and Organs Degeneration, Institute of Biomedicine and Biotechnology, Shenzhen Institutes of Advanced Technology Chinese Academy of Sciences, Shenzhen, 518055, PR China.
Department of Orthopedic Surgery, The First Affiliated Hospital of Nanchang University, Nanchang, 330006, PR China.
Carbohydr Polym. 2020 Dec 1;249:116826. doi: 10.1016/j.carbpol.2020.116826. Epub 2020 Aug 3.
Wound dressings based on injectable thermo-sensitive hydrogel possess several advantages over preformed conventional dressings such as rapid reversible sol-gel transition behavior and the capacity of filling the irregular wound defect. Nevertheless, its clinical application is hindered by the weak tissue adhesiveness. Therefore, in this study, the catechol modified quaternized chitosan (QCS-C) was fabricated and incorporated into poly(d,l-lactide)-poly(ethylene glycol)-poly(d,l-lactide) (PLEL) hydrogel to develop an injectable hydrogel with the properties of thermo-sensitive, antibacterial and tissue adhesive. QCS-C could lower the LCST of hydrogel for easy gelation at physiological temperature, and significantly enhanced the tissue adhesion. For wound generation, nano-scaled bioactive glass (nBG：80 SiO, 16 CaO and 4 PO; mol%) was loaded into hydrogel to promote angiogenesis. The mice partial laceration experiment showed that PLEL-nBG-QCS-C hydrogel could effectively seal the ruptured skin and significantly accelerate wound healing. Thus, our findings established a new type of clinical treatment technology for complicated wounds.
基于可注射温敏水凝胶的伤口敷料具有优于预制常规敷料的几个优点，如快速可逆的溶胶-凝胶转变行为和填充不规则伤口缺陷的能力。然而，其临床应用受到组织粘附力弱的阻碍。因此，在本研究中，制备了儿茶酚改性季铵化壳聚糖（QCS-C）并将其掺入聚（d，l-乳酸）-聚乙二醇-聚（d，l-乳酸）（PLEL）水凝胶中，以开发具有温敏、抗菌和组织粘附性能的可注射水凝胶。QCS-C 可以降低水凝胶的 LCST，使其在生理温度下易于凝胶化，并显著增强组织粘附性。为了产生伤口，将纳米级生物活性玻璃（nBG：80SiO、16CaO 和 4PO；摩尔%）负载到水凝胶中以促进血管生成。小鼠部分撕裂实验表明，PLEL-nBG-QCS-C 水凝胶可有效封闭破裂的皮肤并显著加速伤口愈合。因此，我们的研究结果为复杂伤口建立了一种新型的临床治疗技术。
