Cui Hua, Shu Haiyang, Fan Dancai, Wang Xinyu, Zhao Ning, Lu Cheng, Lu Aiping, He Xiaojuan
Institute of Basic Research in Clinical Medicine, China Academy of Chinese Medical Sciences, Beijing 100700, China.
School of Life Science and Engineering, Southwest Jiaotong University, Chengdu 611756, China.
Evid Based Complement Alternat Med. 2020 Mar 19;2020:1015083. doi: 10.1155/2020/1015083. eCollection 2020.
Wang-Bi Capsule (WB), a traditional Chinese medicine- (TCM-) based herbal formula, is currently used in clinic for the treatment of rheumatoid arthritis (RA) with positive clinical effects. However, its pharmacological mechanism of action in RA is still obscure. Therefore, this study established a collagen-induced arthritis (CIA) mice model to examine the efficacy of WB by using arthritis score, histological analysis, and micro-CT examination. Proinflammatory cytokines expression, osteoclast number, OPG/RANKL system, and NF-B activation were then detected to further investigate the mechanism of WB in RA treatment. The results indicated that WB could alleviate the erythema and swelling of paws in CIA mice. It also inhibited the infiltration of inflammatory cells and bone destruction and increased bone density in joints of CIA mice. Mechanistic studies showed that WB treatment decreased the production of IL-1, IL-6, and TNF- in serum and joints of CIA mice. Moreover, it reduced the osteoclast number, increased OPG level, decreased RANKL level, and inhibited the activation of NF-B in joints of CIA mice. In conclusion, this study demonstrated that WB could effectively alleviate disease progression of CIA mice by decreasing the IL-1, IL-6, and TNF- levels, modulating the OPG/RANKL system, and inhibiting the activation of NF-B.
尪痹胶囊（WB）是一种基于传统中药的草药配方，目前在临床上用于治疗类风湿关节炎（RA），临床效果良好。然而，其在RA中的药理作用机制仍不清楚。因此，本研究建立了胶原诱导性关节炎（CIA）小鼠模型，通过关节炎评分、组织学分析和显微CT检查来检测WB的疗效。随后检测促炎细胞因子表达、破骨细胞数量、OPG/RANKL系统和NF-κB激活情况，以进一步研究WB治疗RA的机制。结果表明，WB可减轻CIA小鼠爪部的红斑和肿胀。它还抑制了CIA小鼠关节中炎性细胞的浸润和骨质破坏，并增加了骨密度。机制研究表明，WB治疗降低了CIA小鼠血清和关节中IL-1、IL-6和TNF-α的产生。此外，它减少了CIA小鼠关节中的破骨细胞数量，增加了OPG水平，降低了RANKL水平，并抑制了NF-κB的激活。总之，本研究表明，WB可通过降低IL-1、IL-6和TNF-α水平、调节OPG/RANKL系统以及抑制NF-κB的激活来有效减轻CIA小鼠的疾病进展。
