Huang Shaoyan, Han Shan, Zhang Jianzhong, Zhong Zhaokun, Wang Jianrong
Department of Oncology, Yantaishan Hospital, Yantai, Shandong 264000, P.R. China.
Department of Oncology, Dongying People's Hospital, Dongying, Shandong 257091, P.R. China.
Oncol Lett. 2020 Apr;19(4):3333-3338. doi: 10.3892/ol.2020.11444. Epub 2020 Mar 3.
The present retrospective study aimed to investigate the expression of semaphorin-4C (Sema4C) in epithelial ovarian cancer (EOC), and to determine the association between Sema4C expression and patient clinicopathological characteristics. Sema4C mRNA expression was detected by reverse transcription-quantitative polymerase chain reaction in the tissues of 74 cases of EOC, 20 cases of ovarian epithelial benign tumor, 20 cases of ovarian borderline epithelial tumor and 15 cases of normal ovarian tissue. Immunohistochemistry was used to detect the expression and localization of Sema4C. The association between Sema4C expression level and patients clinicopathological characteristics was determined by χ test. The results demonstrated that Sema4C expression level in ovarian epithelial carcinoma tissues was significantly higher compared with that in benign tumors, borderline epithelial tumors and normal ovarian tissues (P<0.05). In addition, Sema4C expression in ovarian cancer tissues was significantly associated with the clinical and pathological stages of tumors (P<0.05). In conclusion, the present study demonstrated that Sema4C expression was upregulated in EOC.
本回顾性研究旨在探讨信号素4C（Sema4C）在上皮性卵巢癌（EOC）中的表达情况，并确定Sema4C表达与患者临床病理特征之间的关联。采用逆转录-定量聚合酶链反应检测74例EOC组织、20例卵巢上皮良性肿瘤组织、20例卵巢交界性上皮肿瘤组织及15例正常卵巢组织中Sema4C mRNA的表达。采用免疫组织化学法检测Sema4C的表达及定位。采用χ检验确定Sema4C表达水平与患者临床病理特征之间的关联。结果显示，卵巢上皮癌组织中Sema4C表达水平显著高于良性肿瘤、交界性上皮肿瘤及正常卵巢组织（P<0.05）。此外，卵巢癌组织中Sema4C表达与肿瘤的临床及病理分期显著相关（P<0.05）。综上所述，本研究表明EOC中Sema4C表达上调。
