Nano-graphene oxide with antisense RNA inhibits the pathogenicity of in periapical periodontitis.

BACKGROUND/PURPOSE: () is considered a predominant pathogen for persistent periapical infections. Antisense (AS) RNA was reported to inhibit the biofilm formation and sensitized to calcium hydroxide medication. The aims of this study were to investigate whether the graphene oxide (GO) nanosheets could be used to enhance antibacterial activity of AS RNA for in periapical periodontitis.

MATERIALS AND METHODS

We developed a graphene-based plasmid transformation system by loading antisense plasmid with GO-polyethylenimine (PEI) complexes (GO-PEI-AS). The particle size distributions and zeta-potential of the GO-PEI-AS were evaluated. Then, AS plasmids were labeled with gene encoding enhanced green fluorescent protein (ASR-eGFP). The transformation efficiencies and the bacterial viability of were evaluated by confocal laser scanning microscopy. Quantitative real-time PCR assays were used to investigate the expressions of virulent genes after transformed by GO-PEI-AS. Also, the antibacterial properties of the GO-PEI-AS were validated in the rat periapical periodontitis model.

RESULTS

We showed that GO-PEI could efficiently deliver the AS plasmid into cell. GO-PEI-AS significantly reduced virulent-associated gene expressions. Furthermore, GO-PEI-AS suppressed biofilm aggregation and improved bactericidal effects using infected canal models . In four-weeks periapical infective rat models, the GO-PEI-AS strains remarkably reduced the periapical lesion size.

CONCLUSION

Transformation efficiency and antibacterial prosperity of AS can be marked improved by GO-PEI based delivery system for infections.