Nawaz Afrah, Ali Syed Mohsin, Rana Nosheen Fatima, Tanweer Tahreem, Batool Amna, Webster Thomas J, Menaa Farid, Riaz Sundus, Rehman Zahra, Batool Farhat, Fatima Misha, Maryam Tuba, Shafique Iqra, Saleem Abida, Iqbal Arfa
Department of Biomedical Engineering and Sciences, School of Mechanical & Manufacturing Engineering, National University of Science & Technology, Islamabad 44000, Pakistan.
Department of Chemical Engineering, Northeastern University, Boston, MA 02115, USA.
Nanomaterials (Basel). 2021 Nov 22;11(11):3152. doi: 10.3390/nano11113152.
Metallic nanoparticles, such as gold nanoparticles (AuNPs), have been extensively studied as drug delivery systems for various therapeutic applications. However, drug-loaded-AuNPs have been rarely explored in vivo for their effect on bacteria residing inside tissues. Ciprofloxacin (CIP) is a second-generation fluoroquinolone with a broad-spectrum of antibiotic properties devoid of developing bacteria resistance. This research is focused on the synthesis and physical characterization of Ciprofloxacin-loaded gold nanoparticles (CIP-AuNPs) and their effect on the colonization of in the liver and kidneys of mice. The successfully prepared CIP-AuNPs were stable and exerted enhanced in vitro antibacterial activity against compared with free CIP. The optimized CIP-AuNPs were administered (500 µg/Kg) once a day via tail vein to infected mice for eight days and were found to be effective in eradicating from the host tissues. Moreover, unlike CIP, CIP-AuNPs were non-hemolytic. In summary, this study demonstrated that CIP-AuNPs are promising and biocompatible alternative therapeutics for -induced infections resistant to conventional drugs (e.g., beta-lactams and vancomycin) and should be further investigated.
金属纳米颗粒，如金纳米颗粒（AuNPs），作为用于各种治疗应用的药物递送系统已被广泛研究。然而，负载药物的金纳米颗粒对组织内细菌的体内作用却鲜有探索。环丙沙星（CIP）是第二代氟喹诺酮类药物，具有广谱抗菌特性且不易产生细菌耐药性。本研究聚焦于负载环丙沙星的金纳米颗粒（CIP-AuNPs）的合成与物理表征及其对小鼠肝脏和肾脏中[此处原文缺失相关细菌名称]定植的影响。成功制备的CIP-AuNPs稳定，与游离环丙沙星相比，对[此处原文缺失相关细菌名称]具有更强的体外抗菌活性。优化后的CIP-AuNPs以500 µg/Kg的剂量通过尾静脉每天给药一次，持续八天，用于感染小鼠，结果发现其能有效清除宿主组织中的[此处原文缺失相关细菌名称]。此外，与环丙沙星不同，CIP-AuNPs无溶血作用。总之，本研究表明CIP-AuNPs是针对对传统药物（如β-内酰胺类和万古霉素）耐药的[此处原文缺失相关细菌名称]诱导感染的有前景且生物相容性良好的替代疗法，应进一步研究。
