Department of Nanobiotechnology, Institute of Biology, Warsaw University of Life Sciences, Warsaw, 02-786, Poland.
Centre for Advanced Materials and Technologies CEZAMAT, Warsaw University of Technology, Warsaw, 02-822, Poland.
Int J Nanomedicine. 2023 Aug 28;18:4839-4855. doi: 10.2147/IJN.S419957. eCollection 2023.
Graphene oxide (GO) is a single layer of carbon atoms with unique properties, which are beneficial due to its surface functionalisation by miRNA. miRNAs are a non-coding small form of RNA that suppress the expression of protein-coding genes by translational repression or degradation of messenger RNA. Antisense miRNA-21 is very promising for future investigation in cancer therapy. This study aimed to detect cytokine expression levels after the administration of GO-antisense miRNA-21 into U87, U118, U251 and T98 glioma cell lines.
U87, U118, U251 and T98 glioma cell line were investigated in term of viability, human cytokine expression level at protein and genes after treatment with GO, GO-antisense miRNA-21 and antisense miRNA-21. The delivery of antisense miRNA-21 into the glioma cell at in vitro investigation were conducted by GO based transfection and electroporation.
The results of the protein microarray and gene expression profile showed that complexes of GO-antisense miRNA-21 modified the metallopeptidase inhibitor 2 (TIMP-2), interleukin-6 (IL-6), interleukin 8 (IL-8), intercellular adhesion molecule 1 (ICAM-1), and monocyte chemoattractant protein-1 (MCP-1) expression level compared to transfection by electroporation of antisense miRNA-21 at investigated glioblastoma cell lines. The TIMP-2 protein and gene expression level was upregulated after antisense miRNA-21 delivery by GO complex into U87, U251 and T98 glioblastoma cell lines comparing to the non-treated control group. The downregulation at protein expression level of ICAM - 1 was observed at U87, U118, U251 and T98 glioma cell lines. Moreover, the IL-8 expression level at mRNA for genes and protein was decreased significantly after delivery the antisense-miRNA-21 by GO compared to electroporation as a transfection method.
This work demonstrated that the graphene oxide complexes with antisense miRNA-21 can effectively modulate the cytokine mRNA and protein expression level at U87, U118, U251 and T98 glioma cell lines.
氧化石墨烯（GO）是单层碳原子，具有独特的性质，由于其通过 miRNA 进行表面功能化，因此具有有益的性质。miRNA 是一种非编码的小 RNA 形式，可以通过翻译抑制或信使 RNA 的降解来抑制蛋白质编码基因的表达。反义 miRNA-21 在癌症治疗的未来研究中非常有前景。本研究旨在检测 GO-反义 miRNA-21 给药后 U87、U118、U251 和 T98 神经胶质瘤细胞系中细胞因子表达水平。
研究了 U87、U118、U251 和 T98 神经胶质瘤细胞系在 GO、GO-反义 miRNA-21 和反义 miRNA-21 处理后的细胞活力、人类细胞因子表达水平、蛋白质和基因水平。通过基于 GO 的转染和电穿孔将反义 miRNA-21 递送至神经胶质瘤细胞系进行体外研究。
蛋白质微阵列和基因表达谱的结果表明，与电穿孔转染反义 miRNA-21 相比，GO-反义 miRNA-21 修饰了金属肽酶抑制剂 2（TIMP-2）、白细胞介素 6（IL-6）、白细胞介素 8（IL-8）、细胞间黏附分子 1（ICAM-1）和单核细胞趋化蛋白 1（MCP-1）的表达水平。与未经处理的对照组相比，GO 复合物转染 U87、U251 和 T98 神经母细胞瘤细胞系后，TIMP-2 蛋白和基因表达水平上调。在 U87、U118、U251 和 T98 神经胶质瘤细胞系中观察到 ICAM-1 蛋白表达水平下调。此外，与电穿孔作为转染方法相比，GO 转染后，IL-8 基因和蛋白的表达水平显著降低。
本工作表明，氧化石墨烯与反义 miRNA-21 复合物可有效调节 U87、U118、U251 和 T98 神经胶质瘤细胞系的细胞因子 mRNA 和蛋白表达水平。
Zotero 插件
