Noteborn H P, Ebels I, Reinharz A C, Pévet P, Benson B, Salemink C A
Department of Organic Chemistry, University of Utrecht, The Netherlands.
J Pineal Res. 1988;5(6):573-87. doi: 10.1111/j.1600-079x.1988.tb00800.x.
The milk-ejecting response of lactating mouse mammary gland tissue to ovine pineal extracts indicated the presence of a neurohormone-like bioactivity in this tissue. After successive fractionation on gel permeation chromatography and reversed-phase liquid chromatography (HPLC) in conjunction with radioimmunoassays (RIA), it was demonstrated that the milk-ejection response to ovine pineal components with an Mr less than 1,000 corresponded to a biologically active peptide sequence that probably differs from that of arginine vasopressin, arginine vasotocin, and oxytocin and from peptides with a COOH-terminal Pro-Arg-Gly-amide ending. Gel permeation chromatography in formic acid appeared also to indicate the presence of a noncovalent interaction of the neurohormone-like bioactivity with proteins (Mr greater than 25,000) of the pineal.
泌乳小鼠乳腺组织对绵羊松果体提取物的排乳反应表明该组织中存在一种神经激素样生物活性。在凝胶渗透色谱和反相液相色谱（HPLC）上连续分级分离并结合放射免疫测定（RIA）后，结果表明，对分子量小于1000的绵羊松果体成分的排乳反应对应于一个生物活性肽序列，该序列可能不同于精氨酸加压素、精氨酸催产素和催产素，也不同于具有COOH末端Pro-Arg-Gly-酰胺结尾的肽。在甲酸中进行的凝胶渗透色谱似乎也表明神经激素样生物活性与松果体中分子量大于25000的蛋白质存在非共价相互作用。
