采用反相液相色谱法和放射免疫分析法对绵羊松果体中的神经垂体激素样肽进行研究。
Neurohypophyseal hormone-like peptides in the ovine pineal gland using reverse-phase liquid chromatography and radioimmunoassay.
作者信息
Noteborn H P, Reinharz A C, Pévet P, Ebels I, Salemink C A
机构信息
Department of Organic Chemistry, University of Utrecht, The Netherlands.
出版信息
Peptides. 1988 May-Jun;9(3):455-62. doi: 10.1016/0196-9781(88)90147-7.
A method is described for the determination of the neurohormone contents of ovine pineal tissue by radioimmunoassay (RIA) after successive fractionation on gel filtration in formic acid and reverse-phase liquid chromatography (HPLC). This method gives a good resolution for the neurohormones vasopressin, vasotocin and oxytocin, without a significant interference of aspecific cross-reacting of peptides with the RIA. An acid extract from ovine pineal tissue was found to contain amounts of immunoreactive AVP- and OXT-like peptides, whereas an AVT-like peptide was not detectable over background levels after HPLC with post-column RIA. It is concluded from our results that an AVT-like peptide is not present in ovine pineal tissue, and the pineal AVP- and OXT-like peptides appeared to be associated to neurophysin molecules.
本文描述了一种通过放射免疫分析法（RIA）测定绵羊松果体组织神经激素含量的方法，该方法先在甲酸中进行凝胶过滤，然后进行反相液相色谱（HPLC）连续分级分离。该方法对神经激素血管加压素、加压催产素和催产素具有良好的分辨率，且肽类与RIA的非特异性交叉反应干扰不显著。发现绵羊松果体组织的酸提取物中含有一定量的免疫反应性抗利尿激素（AVP）样肽和催产素（OXT）样肽，而在HPLC柱后RIA检测中，未检测到高于背景水平的加压催产素（AVT）样肽。从我们的结果可以得出结论，绵羊松果体组织中不存在AVT样肽，松果体中的AVP样肽和OXT样肽似乎与神经垂体激素运载蛋白分子有关。
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