Larsen B R, Findell P R, Benson B
Prog Clin Biol Res. 1982;92:117-27.
We have initiated a project designed to isolate and identify the neurohypophysial peptides found in the bovine pineal gland. Our principle interest is to provide definitive molecular evidence in order to eliminate the conflict created by the present literature as to the presence of arginine vasotocin in the mammalian pineal gland. The pineals were extracted and chromatographed according to standard methods for the isolation of neurohypophysial peptides. All fractions were assayed for their ability to cause milk ejection using the in vivo assay described by Hadley et al. (1975). However, during the course of the isolation procedure a partially purified fraction was isolated that showed neurohypophysial hormone-like activity. According to tests run on parallel chromatographic columns, the substance responsible for the neurohypophysial hormone-like activity could not be oxytocin, arginine vasotocin or arginine vasopressin.
我们启动了一个项目，旨在分离和鉴定牛松果体中发现的神经垂体肽。我们主要的兴趣在于提供确凿的分子证据，以消除当前文献中关于哺乳动物松果体中是否存在精氨酸血管催产素所产生的冲突。按照分离神经垂体肽的标准方法对松果体进行提取和色谱分析。使用哈德利等人（1975年）描述的体内试验，对所有级分引起排乳的能力进行测定。然而，在分离过程中，分离出了一个部分纯化的级分，该级分显示出神经垂体激素样活性。根据在平行色谱柱上进行的测试，具有神经垂体激素样活性的物质不可能是催产素、精氨酸血管催产素或精氨酸加压素。
