Wang Qian, Zhang Yangdong, Zheng Nan, Zhao Shengguo, Li Songli, Wang Jiaqi
Chinese Academy of Agricultural Sciences, State Key Laboratory of Animal Nutrition, Institute of Animal Science, Beijing, People's Republic of China.
Chinese Academy of Agricultural Sciences, Key Laboratory of Quality & Safety Control for Milk and Dairy Products of Ministry of Agriculture and Rural Affairs, Institute of Animal Sciences, Beijing, People's Republic of China.
PeerJ. 2020 Mar 26;8:e8742. doi: 10.7717/peerj.8742. eCollection 2020.
Previous studies on the effects of mycotoxins have solely focused on their biochemical profiles or products in dairy ruminants. Changes in metabolism that occur after exposure to mycotoxins, as well as biochemical changes, have not been explored.
We measured the biochemical and metabolic changes in dairy cows after exposure to mycotoxins using biochemical analyses and nuclear magnetic resonance. Twenty-four dairy cows were randomly assigned to three different treatment groups. Control cows received diets with 2 kg uncontaminated cottonseed. Cows in the 50% replacement group received the same diet as the control group, but with 1 kg of uncontaminated cottonseed and 1 kg of cottonseed contaminated with mycotoxins. Cows in the 100% replacement group received the same diet as the control, but with 2 kg contaminated cottonseed.
The results showed that serum γ-glutamyl transpeptidase and total antioxidant capacities were significantly affected by cottonseed contaminated with mycotoxins. There were also significant differences in isovalerate and NH-N levels, and significant differences in the eight plasma metabolites among the three groups. These metabolites are mainly involved in amino acid metabolism pathways. Therefore, the results suggest that amino acid metabolism pathways may be affected by mycotoxins exposure.
先前关于霉菌毒素影响的研究仅关注其在反刍动物乳制品中的生化特征或产物。接触霉菌毒素后发生的代谢变化以及生化变化尚未得到探索。
我们通过生化分析和核磁共振测量了奶牛接触霉菌毒素后的生化和代谢变化。将24头奶牛随机分为三个不同的处理组。对照组奶牛饲喂含2千克未受污染棉籽的日粮。50%替代组的奶牛接受与对照组相同的日粮，但含有1千克未受污染棉籽和1千克受霉菌毒素污染的棉籽。100%替代组的奶牛接受与对照组相同的日粮，但含有2千克受污染棉籽。
结果表明，受霉菌毒素污染的棉籽对血清γ-谷氨酰转肽酶和总抗氧化能力有显著影响。异戊酸和NH-N水平也存在显著差异，三组之间的八种血浆代谢物也存在显著差异。这些代谢物主要参与氨基酸代谢途径。因此，结果表明氨基酸代谢途径可能受到霉菌毒素暴露的影响。
