Bodas Raúl, Giráldez F Javier, Olmedo Sara, Herrera Marta, Lorán Susana, Ariño Agustín, López Secundino, Benito Alberto, Juan Teresa
Agrarian Technological Institute of Castile and Leon (ITACyL), Ctra. Burgos, Km 119, 47071 Valladolid, Spain.
Instituto de Ganadería de Montaña (CSIC-Universidad De León), Finca Marzanas s/n, 24346 Grulleros, Spain.
Animals (Basel). 2023 Jan 27;13(3):436. doi: 10.3390/ani13030436.
The aim of this study was to investigate the in vivo transfer of aflatoxin B1 (AFB1) to Assaf ewes' milk (aflatoxin M1, AFM1) and its effect on animal performance and health. Thirty Assaf ewes were allocated to three groups (C, L, H), and received a different individual daily dose of AFB1 (0, 40 and 80 μg) for 13 days. Milk (days 1, 2, 3, 4, 7, 14, 16 and 18) and blood (days 1, 7, 14 and 18) samples were collected. Milk yield, composition (except protein) and somatic cell counts (SCC) were not affected by AFB1 intake ( > 0.05). Haemoglobin concentration increased ( < 0.05) and haematocrit and alanine aminotransferase levels tended to increase ( < 0.10) in group H on day 14. AFM1 excretion was highly variable and detected in L and H animals from days 1 to 16 (3 days increase, 10 days steady-state, 3 days clearance). Carry-over rate (0.23%) was significantly higher in L (0.22-0.34%) than in H (0.16-0.19%) animals ( < 0.05). AFB1 daily doses of 40 to 80 µg do not impair milk yield; however, it may start affecting animals' health. Milk AFM1 depends mainly on the AFB1 intake whereas carryover rate is positively influenced by the level of milk production.
本研究的目的是调查黄曲霉毒素B1（AFB1）在体内向阿萨夫母羊乳汁（黄曲霉毒素M1，AFM1）的转移情况及其对动物生产性能和健康的影响。将30只阿萨夫母羊分为三组（C、L、H），连续13天分别给予不同的AFB1每日个体剂量（0、40和80微克）。采集乳汁（第1、2、3、4、7、14、16和18天）和血液（第1、7、14和18天）样本。AFB1摄入量对产奶量、成分（蛋白质除外）和体细胞计数（SCC）没有影响（P>0.05）。第14天，H组的血红蛋白浓度升高（P<0.05），血细胞比容和丙氨酸转氨酶水平有升高趋势（P<0.10）。AFM1排泄高度可变，在第1至16天在L组和H组动物中检测到（3天上升期、10天稳态期、3天清除期）。L组（0.22 - 0.34%）的残留率（0.23%）显著高于H组（0.16 - 0.19%）动物（P<0.05）。每日40至80微克的AFB1剂量不会损害产奶量；然而，它可能开始影响动物健康。乳汁中的AFM1主要取决于AFB1摄入量，而残留率则受到产奶水平正影响。
