Choi Jun Young, Kim So Hyun, Kim Ji Eun, Park Ji Won, Kang Mi Ju, Choi Hyeon Jun, Bae Su Ji, Lee Jae Ho, Jung Young-Suk, Hwang Dae Youn
1Department of Biomaterials Science, College of Natural Resources and Life Science, Pusan National University, 50 Cheonghak-ri, Samnangjin-eup Miryang-si, Gyeongsangnam-do 50463 South Korea.
2College of Pharmacy, Pusan National University, Busan, 46241 South Korea.
Lab Anim Res. 2019 Dec 30;35:32. doi: 10.1186/s42826-019-0033-x. eCollection 2019.
The butanol extract of roots fermented with (BAW) effectively prevents inflammation and remodeling of airway in the ovalbumin (OVA)-induced asthma model. To characterize biomarkers that can predict the anti-asthmatic effects induced by BAW treatment, we measured the alteration of endogenous metabolites in the serum of OVA-induced asthma mice after administration of low concentration BAW (BAWLo, 250 mg/kg) and high concentration BAW (BAWHi, 500 mg/kg) using H nuclear magnetic resonance (H-NMR) spectral data. The number of immune cells and serum concentration of IgE as well as thickness of the respiratory epithelium and infiltration of inflammatory cells in the airway significantly recovered in the OVA+BAW treated group as compared to the OVA+Vehicle treated group. In the metabolic profile analysis, the pattern recognition showed completely separate clustering of serum analysis parameters between the OVA+Vehicle and OVA+BAW treated groups. Of the total endogenous metabolites, 19 metabolites were upregulated or downregulated in the OVA+Vehicle treated group as compared to the Control treated group. However, only 4 amino acids (alanine, glycine, methionine and tryptophan) were significantly recovered after BAWLo and BAWHi treatment. This study provides the first results pertaining to metabolic changes in the asthma model mice treated with OVA+BAW. Additionally, these findings show that 4 metabolites can be used as one of biomarkers to predict the anti-asthmatic effects.
用（BAW）发酵的根的丁醇提取物可有效预防卵清蛋白（OVA）诱导的哮喘模型中的气道炎症和重塑。为了鉴定能够预测BAW治疗诱导的抗哮喘作用的生物标志物，我们使用氢核磁共振（H-NMR）光谱数据，测量了低浓度BAW（BAWLo，250mg/kg）和高浓度BAW（BAWHi，500mg/kg）给药后OVA诱导的哮喘小鼠血清中内源性代谢物的变化。与OVA+载体处理组相比，OVA+BAW处理组中免疫细胞数量、IgE血清浓度以及呼吸道上皮厚度和气道中炎症细胞浸润均显著恢复。在代谢谱分析中，模式识别显示OVA+载体处理组和OVA+BAW处理组之间血清分析参数完全分开聚类。与对照处理组相比，OVA+载体处理组中19种内源性代谢物上调或下调。然而，BAWLo和BAWHi处理后只有4种氨基酸（丙氨酸、甘氨酸、蛋氨酸和色氨酸）显著恢复。本研究提供了关于OVA+BAW处理的哮喘模型小鼠代谢变化的首个结果。此外，这些发现表明4种代谢物可作为预测抗哮喘作用的生物标志物之一。
