HBV 相关肝细胞癌患者髓源性抑制细胞表达增加。

Increased Expression of Myeloid-Derived Suppressor Cells in Patients with HBV-Related Hepatocellular Carcinoma.

机构信息

Graduate College of Hebei Medical University, Hebei Medical University, Shijiazhuang, Hebei 050017, China.

College of Life Sciences, Hebei Normal University, Shijiazhuang, Hebei 050024, China.

出版信息

Biomed Res Int. 2020 Mar 14;2020:6527192. doi: 10.1155/2020/6527192. eCollection 2020.

METHODS

The percentages of MDSCs, IFN--producing CD4 and CD8 T cells in the peripheral blood of HCC patients, chronic hepatitis B (CHB) patients, and healthy controls (HC) were determined by flow cytometry. The serum concentrations of IL-10 and TNF- were determined by ELISA. The association of the percentages of MDSCs with tumor burden, liver function parameters, systemic inflammation-related indexes, and IFN--producing T cells was assessed.

RESULTS

The percentages of MDSCs and PMN-MDSCs were significantly higher in HCC patients than those in CHB patients and HC. The level of MDSCs was correlated with indirect bilirubin and prealbumin, as well as systemic inflammation response index, monocyte/lymphocyte ratio, and monocyte counts. The frequency of IFN--producing CD8 T cells of HCC patients was lower than that of HC. However, there was no relationship between MDSCs and IFN--producing CD8 T cells. The level of IL-10 in HCC patients was significantly higher than that in CHB patients.

CONCLUSION

MDSCs seem to play an important role in the process leading from chronic HBV infection to HCC. Early inhibiting these cells could affect tumor progression.

方法

采用流式细胞术检测 HCC 患者、慢性乙型肝炎（CHB）患者和健康对照者（HC）外周血中髓系抑制细胞（MDSCs）、IFN--产生的 CD4 和 CD8 T 细胞的百分比。采用 ELISA 法检测血清中白细胞介素-10（IL-10）和肿瘤坏死因子-α（TNF-α）的浓度。评估 MDSCs 百分比与肿瘤负荷、肝功能参数、全身炎症相关指标和 IFN--产生的 T 细胞之间的相关性。

结果

与 CHB 患者和 HC 相比，HCC 患者的 MDSCs 和 PMN-MDSCs 百分比显著升高。MDSCs 水平与间接胆红素和前白蛋白以及全身炎症反应指数、单核细胞/淋巴细胞比值和单核细胞计数相关。HCC 患者 IFN--产生的 CD8 T 细胞的频率低于 HC。然而，MDSCs 与 IFN--产生的 CD8 T 细胞之间没有关系。HCC 患者的 IL-10 水平明显高于 CHB 患者。