State Key Laboratory of Oral Diseases, National Clinical Research Center for Oral Diseases, West China School of Stomatology, Sichuan University, Chengdu, 610065, PR China.
Department of Food Engineering, College of Biomass Science and Engineering, Sichuan University, Chengdu, 610064, PR China.
J Ethnopharmacol. 2020 Jul 15;257:112856. doi: 10.1016/j.jep.2020.112856. Epub 2020 Apr 9.
Cedrus deodara (Roxb. ex D.Don) G. Don is applied as anti-inflammatory and anti-infection agents in folklore medicine.
The present study aimed to assess the antimicrobial activity of Cedrus deodara (Roxb. ex D.Don) G. Don extract (CDE) against Streptococcus mutans biofilm formation and its biocompatibility, as well as to identify its chemical components.
Confocal laser scanning microscopy (CLSM), crystal violet staining, and CFU counting assay were applied to investigate the effect of CDE on S. mutans biofilm formation and extracellular polysaccharides (EPS) synthesis. The microstructure of S. mutans biofilms formed on glass coverslips and bovine enamel treated with CDE was observed by scanning electron microscopy (SEM). qRT-PCR was used to measure the expression of virulence genes gtfB, gtfC, and gtfD, and zymogram assay was performed to investigate the enzymatic activity of Gtfs. Moreover, HPLC-MS and NMR were applied to identify its chemical components. CCK-8 assay was also performed on human oral cells to evaluate its biocompatibility.
Under the treatment of CDE, S. mutans formed less biofilm on both coverslips and enamel surfaces and synthesized less EPS. Moreover, CDE downregulated the expression of gtf genes and inhibited the enzymatic activity of Gtfs. According to HPLC-MS and NMR results, molecular structures of six main compounds in CDE were identified. CDE also has a good biocompatibility.
CDE exhibits inhibitory activity against S. mutans and a good biocompatibility. It has the potential to be developed as anti-caries agents for clinical use.
雪松（Roxb. ex D.Don）G. Don 被民间医学用作抗炎和抗感染药物。
本研究旨在评估雪松提取物（CDE）对变形链球菌生物膜形成的抗菌活性及其生物相容性，并鉴定其化学成分。
采用共聚焦激光扫描显微镜（CLSM）、结晶紫染色和 CFU 计数法研究 CDE 对变形链球菌生物膜形成和细胞外多糖（EPS）合成的影响。用扫描电子显微镜（SEM）观察 CDE 处理的玻璃载玻片和牛牙釉质上变形链球菌生物膜的微观结构。用 qRT-PCR 测量毒力基因 gtfB、gtfC 和 gtfD 的表达，用酶谱法研究 Gtfs 的酶活性。此外，还采用 HPLC-MS 和 NMR 鉴定其化学成分。还用人口腔细胞的 CCK-8 测定法评估其生物相容性。
在 CDE 处理下，变形链球菌在载玻片和牙釉质表面形成的生物膜较少，合成的 EPS 也较少。此外，CDE 下调了 gtf 基因的表达并抑制了 Gtfs 的酶活性。根据 HPLC-MS 和 NMR 结果，鉴定了 CDE 中六种主要化合物的分子结构。CDE 还具有良好的生物相容性。
CDE 对变形链球菌具有抑制活性和良好的生物相容性。它有可能被开发为临床用抗龋药物。
