Department of Endocrinology and Metabolism, The First Hospital of Jilin University, 130061 Changchun, Jilin Province, China.
Key Laboratory of Organ Regeneration & Transplantation of the Ministry of Education, The First Hospital of Jilin University, 130061 Changchun, Jilin Province, China.
J Diabetes Res. 2020 Mar 18;2020:7152176. doi: 10.1155/2020/7152176. eCollection 2020.
20(S)-Ginsenoside Rg3 (20(S)-Rg3) has been shown to induce apoptosis by interfering with several signaling pathways. Furthermore, it has been reported to have anticancer and antidiabetic effects. In order to detect the protective effect of 20(S)-Rg3 on diabetic kidney disease (DKD), diabetic rat models which were established by administering high-sugar, high-fat diet combined with intraperitoneal injection of streptozotocin (STZ), and age-matched wild-type (WT) rat were given 20(S)-Rg3 for 12 weeks, with three groups: control group (normal adult rats with saline), diabetic group (diabetic rats with saline), and 20(S)-Rg3 treatment group (diabetic rats with 20(S)-Rg3 (10 mg/kg body weight/day)). The biochemical indicators and the changes in glomerular basement membrane and mesangial matrix were detected. TUNEL staining was used to detect glomerular and renal tubular cell apoptosis. Immunohistochemical staining was used to detect the expression of fibrosis factors and inflammation factors in rat kidney tissues. Through periodic acid-Schiff staining, we observed that the change in renal histology was improved and renal tubular epithelial cell apoptosis decreased significantly by treatment with 20(S)-Rg3. Plus, the urine protein decreased in the rats with the 20(S)-Rg3 treatment. Fasting blood glucose, creatinine, total cholesterol, and triglyceride levels in the 20(S)-Rg3 treatment group were all lower than those in the diabetic group. Mechanistically, 20(S)-Rg3 dramatically downregulated the expression of TGF-1, NF-B65, and TNF- in the kidney. These resulted in a significant prevention of renal damage from the inflammation. The results of the current study suggest that 20(S)-Rg3 could potentially be used as a novel treatment against DKD.
20(S)-人参皂苷 Rg3（20(S)-Rg3）已被证明通过干扰几种信号通路诱导细胞凋亡。此外，它还具有抗癌和抗糖尿病作用。为了检测 20(S)-Rg3 对糖尿病肾病（DKD）的保护作用，我们建立了高糖、高脂肪饮食联合腹腔注射链脲佐菌素（STZ）的糖尿病大鼠模型，并给予三种不同处理：对照组（正常成年大鼠用生理盐水）、糖尿病组（糖尿病大鼠用生理盐水）和 20(S)-Rg3 治疗组（糖尿病大鼠用 20(S)-Rg3（10mg/kg 体重/天）），共 12 周。检测生化指标及肾小球基底膜和系膜基质的变化。TUNEL 染色检测肾小球和肾小管细胞凋亡。免疫组化染色检测大鼠肾脏组织中纤维化因子和炎症因子的表达。通过过碘酸希夫染色，我们观察到 20(S)-Rg3 治疗可改善肾脏组织学变化，显著减少肾小管上皮细胞凋亡。此外，20(S)-Rg3 治疗组大鼠尿蛋白减少。20(S)-Rg3 治疗组大鼠空腹血糖、肌酐、总胆固醇和甘油三酯水平均低于糖尿病组。机制上，20(S)-Rg3 显著下调了肾脏中 TGF-1、NF-B65 和 TNF-的表达。这导致炎症引起的肾损伤得到显著预防。本研究结果表明，20(S)-Rg3 可能成为治疗 DKD 的一种新方法。
