van Breemen Richard B
Linus Pauling Institute, Oregon State University, 307 Linus Pauling Science Center, Corvallis, OR 97331, TEL: 541-737-5078; FAX: 541-737-5077.
Clin Mass Spectrom. 2020 Jan;15:25-28. doi: 10.1016/j.clinms.2019.12.001. Epub 2020 Jan 7.
As in drug discovery and development, mass spectrometry has become essential at all stages for establishing the safety and efficacy of botanical dietary supplements. Applications of mass spectrometry to the development of botanical dietary supplements include preclinical studies of the mechanisms of action (., proteomic target identification and validation); identification of active natural products using high resolution tandem mass spectrometry; chemical standardization using UHPLC-MS/MS; and studies of metabolism, absorption and toxicity of active compounds using high resolution and UHPLC-MS/MS. Clinical applications of mass spectrometry include evaluation of the potential for drug-botanical interactions; investigation of the pharmacokinetics of active compounds; and quantitative analysis of biomarkers of efficacy during Phase I and II and clinical trials of safety and efficacy of botanical dietary supplements.
与药物发现和开发一样，质谱分析在确定植物性膳食补充剂的安全性和有效性的各个阶段都已变得至关重要。质谱分析在植物性膳食补充剂开发中的应用包括作用机制的临床前研究（如蛋白质组学靶点鉴定和验证）；使用高分辨率串联质谱鉴定活性天然产物；使用超高效液相色谱-质谱/质谱进行化学标准化；以及使用高分辨率和超高效液相色谱-质谱/质谱研究活性化合物的代谢、吸收和毒性。质谱分析的临床应用包括评估药物与植物相互作用的可能性；研究活性化合物的药代动力学；以及在I期和II期对植物性膳食补充剂的疗效生物标志物进行定量分析以及安全性和有效性的临床试验。
