Kumar Satyender, Dagar Seema, Kumar Pushpander, Singh Jitender, Kumar Sunil, Kumar Dinesh
Institute of Pharmaceutical Sciences, Kurukshetra University, Kurukshetra, India.
Porto Biomed J. 2017 Sep-Oct;2(5):167-169. doi: 10.1016/j.pbj.2017.03.001. Epub 2017 Apr 4.
This study was undertaken to assess the antifertility effect of hydroalcoholic leaves extract of Linn. which is traditionally used by the woman in Rajasthan state of India to regulate the fertility. The antifertility activity of the extract at dose levels (200 and 400 mg/kg, orally) was evaluated in two experimental animal models. The extract was found to be safe up to a dose of 4000 mg/kg of the extract when administered orally. A good antiimplantation (37.13%) activity in female rats was observed at the tested dose level (400 mg/kg). The extract, when administered alone at 200 mg/kg dose to immature female albino rats, enhanced the estrogen level in the serum whereas significantly decreased the estrogen level at 400 mg/kg dose. The extract along with estradiol at dose level of 400 mg/kg significantly ( < 0.01) decreased the level of estrogen, in comparison to standard group rats indicating the antiestrogenic nature of the extract. The antiestrogenic effect of the extract at higher dose (400 mg/kg) might be due to negative feed-back inhibition on anterior pituitary. Preliminary phytochemical screening has revealed the presence of alkaloids, carbohydrates, flavonoids and saponins in the hydroalcoholic leaf extract of the plant. The antifertility effect of the plant might be due to antiimplantation as well as antiestrogenic effect of the extract which in turn might be due to some of the chemical constituents present in the extract. The results shows that hydroalcoholic extract of L. leaves possess significant antifertility activity at 400 mg/kg, thus, justifying the traditional use of this plant in fertility regulation in females.
本研究旨在评估印度拉贾斯坦邦女性传统用于调节生育的[植物名称]水醇提取物的抗生育作用。在两种实验动物模型中评估了该提取物在剂量水平（200和400毫克/千克，口服）下的抗生育活性。口服给予该提取物时，发现其在剂量高达4000毫克/千克时是安全的。在测试剂量水平（400毫克/千克）下，观察到雌性大鼠有良好的抗着床（37.13%）活性。当以200毫克/千克剂量单独给予未成熟雌性白化大鼠时，该提取物提高了血清中的雌激素水平，而在400毫克/千克剂量时显著降低了雌激素水平。与标准组大鼠相比，该提取物与雌二醇在400毫克/千克剂量水平时显著（<0.01）降低了雌激素水平，表明该提取物具有抗雌激素性质。该提取物在较高剂量（400毫克/千克）下的抗雌激素作用可能是由于对垂体前叶的负反馈抑制。初步植物化学筛选表明该植物水醇叶提取物中存在生物碱、碳水化合物、黄酮类化合物和皂苷。该植物的抗生育作用可能归因于提取物的抗着床以及抗雌激素作用，而这反过来可能又归因于提取物中存在的一些化学成分。结果表明，[植物名称]叶的水醇提取物在400毫克/千克时具有显著的抗生育活性，因此，证明了该植物在女性生育调节中的传统用途合理。
