Institute of Pharmaceutical Sciences, Kurukshetra University, Kurukshetra, Haryana, India.
J Ethnopharmacol. 2013 Jun 3;147(3):671-5. doi: 10.1016/j.jep.2013.03.003. Epub 2013 Mar 14.
Michelia champaca L. (family: Magnoliaceae), commonly known as Champa [Hindi], is traditionally used for fertility regulation by the women of Chhattisgarh state in India. No scientific evidence regarding the antifertility effect of this plant is available till date.
To study the anti-fertility effect of hydroalcoholic leaf extract of Michelia champaca Linn. in female rats.
The antifertility activity of the extract (HAEMC) administered at dose levels (100 and 200mg/kg body weight, p.o.) was evaluated in two experimental animal models i.e. antiimplantation activity in female wistar rats and esterogenic/antiestrogenic activity in ovariectomized female rats. In anti-implantation activity, the extract (200 and 400mg/kg body weight, p.o.) was administered to female rats from 1 to 7 days of pregnancy and on 10th day, laprotomy was performed to count the no. of implants. For estrogenic/anti-estrogenic activity, ovariectomized female rats were administered with the extract at both the doses alone as well as along with 17α-ethinyl estradiol (1 μ/rat/day) for 7 consecutive days. On the 8th day, all animals were sacrificed and blood serum was further processed for the estimation of biochemical parameters such as estrogen level, alkaline phosphates, cholesterol, tryglycerides, total protein etc.
The extract (HAEMC) showed significant (p<0.01) 49.95% and 71.03% antiimplantation activities at 100 and 200mg/kg doses respectively. The extract also exhibited significant (p<0.01) estrogenic activity as evidenced by increase in body weight, uterine weight, increased thickness and height of endometrium, vaginal cornification and significant (p<0.01) increase in estrogen, cholesterol, alkaline phosphate and triglycerides levels at higher dose when administered alone as well as along with ethinyl estradiol. Phytochemical screening showed the presence of steroids, flavonoids and alkaloids in the extract.
Hydroalchoholic extract of Michelia champaca leaves possesses significant antifertility effect which might be due to the inhibition of implantation and estrogenic effect which in turn might be due to the presence of some phytoconstituents in the plant.
Michelia champaca L.（木兰科），俗称 Champa [印地语]，传统上被印度恰蒂斯加尔邦的女性用于调节生育能力。迄今为止，尚无关于这种植物的抗生育作用的科学证据。
研究 Michelia champaca Linn. 水醇叶提取物对雌性大鼠的抗生育作用。
在两个实验动物模型中评估提取物（HAEMC）的抗生育活性，即雌性 wistar 大鼠的抗着床活性和去卵巢雌性大鼠的雌激素/抗雌激素活性。在抗着床活性中，从妊娠第 1 天到第 7 天，给雌性大鼠口服（200 和 400mg/kg 体重）提取物，第 10 天剖腹术计算植入物数量。对于雌激素/抗雌激素活性，单独给去卵巢雌性大鼠口服提取物，在两个剂量下以及与 17α-乙炔雌二醇（1μ/大鼠/天）一起连续 7 天。第 8 天，所有动物均被处死，进一步处理血清以用于估计生化参数，如雌激素水平、碱性磷酸酶、胆固醇、甘油三酯、总蛋白等。
提取物（HAEMC）在 100 和 200mg/kg 剂量下分别显示出显著的（p<0.01）49.95%和 71.03%抗着床活性。提取物还表现出显著的雌激素活性，表现为体重、子宫重量增加，子宫内膜厚度和高度增加，阴道角化以及单独给予或与乙炔雌二醇一起给予时雌激素、胆固醇、碱性磷酸酶和甘油三酯水平显著增加（p<0.01）。植物化学筛选表明提取物中存在类固醇、类黄酮和生物碱。
Michelia champaca 叶的水醇提取物具有显著的抗生育作用，这可能是由于抑制着床和雌激素作用所致，而雌激素作用可能是由于植物中存在某些植物成分所致。
