Parvareshi Hamrah Mohsen, Rezaei Tavirani Mostafa, Movahedi Monireh, Ahmadi Karvigh Sanaz
Department of Biochemistry, Faculty of Biological Science, North Tehran Branch, Islamic Azad University, Tehran, Iran.
Proteomics Research Center, School of Allied Medical Sciences, Shahid Beheshti University of Medical Sciences, Tehran, Iran (https://orcid.org/0000-0003-1767-7475).
Arch Acad Emerg Med. 2020 Mar 11;8(1):e18. eCollection 2020.
There is an increasing interest in the use of different biomarkers to help distinguish psychogenic non-epileptic seizure (PNES) from epileptic seizures (ES). This study aimed to evaluate the patterns of differentially expressed serum proteins in ES and PNES cases.
In this cross-sectional study, 4 patients with mesial temporal lobe epilepsy and 4 patients with PNES were selected from patients with history of recurrent seizures. Venous blood samples were obtained within 1 hour after seizure and serum proteomes as well as the extent of protein expression were analyzed.
361 proteins were identified; of these, expression of 197 proteins had altered. 110 (55.9%) proteins were down-regulated and 87 (44.1%) were up-regulated in the PNES samples compared to ES samples. The mean pI for deregulated proteins with 1.5 to 3 fold changes were 6.69 ± 1.68 in proteins with increasing expression in ES group and 5.88 ± 1.39 in proteins with increasing expression in PNES group (p = 0.008). The median and interquartile range (IQR) of molecular weight changes in proteins with 1.5 to 3 fold changes were 64 (22.0-86.0) in proteins whose expression had increased in ES group and 39.5 (26.0-61.5) in proteins whose expression had increased in PNES cases (p = 0.05).
Several spots with differential expression were observed by comparing patients with ES against the PNES groups, which could be potential biomarkers of the disease. Damage to the blood-brain barrier is the most important difference between the two groups, thus identifying total protein changes offers a key to the future of differentiating ES and PNES patients.
人们越来越关注使用不同的生物标志物来帮助区分精神性非癫痫发作（PNES）和癫痫发作（ES）。本研究旨在评估ES和PNES病例中差异表达的血清蛋白模式。
在这项横断面研究中，从有反复发作病史的患者中选取4例内侧颞叶癫痫患者和4例PNES患者。在发作后1小时内采集静脉血样本，分析血清蛋白质组以及蛋白质表达程度。
共鉴定出361种蛋白质；其中，197种蛋白质的表达发生了改变。与ES样本相比，PNES样本中有110种（55.9%）蛋白质表达下调，87种（44.1%）蛋白质表达上调。在ES组中表达增加的蛋白质中，1.5至3倍变化的失调蛋白的平均pI为6.69±1.68，在PNES组中表达增加的蛋白质中为5.88±1.39（p = 0.008）。在ES组中表达增加的蛋白质中，1.5至3倍变化的蛋白质分子量变化的中位数和四分位数间距（IQR）为64（22.0 - 86.0），在PNES病例中表达增加的蛋白质中为39.5（26.0 - 61.5）（p = 0.05）。
通过比较ES患者与PNES组，观察到了几个差异表达的斑点，这些斑点可能是该疾病的潜在生物标志物。血脑屏障受损是两组之间最重要的差异，因此识别总蛋白变化为区分ES和PNES患者的未来提供了关键。
