Parvareshi Hamrah Mohsen, Rezaei Tavirani Mostafa, Movahedi Monireh, Ahmadi Karvigh Sanaz
Department of Biochemistry, Faculty of Biological Science, North Tehran Branch, Islamic Azad University, Tehran, Iran.
Proteomics Research Center, School of Allied Medical Sciences, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Arch Acad Emerg Med. 2020 Oct 29;8(1):e87. eCollection 2020.
Differentiating actual epileptic seizures (ESs) from psychogenic non-epileptic seizures (PNES) is of great interest. This study compares the serum proteomics of patients diagnosed with ESs and PNES.
Eight patients with seizure (4 with PNES and 4 with TLE (temporal lope epilepsy)) were enrolled in this comparative study. Venous blood samples were drawn during the first hour following the seizure. Standard protein purification technique was employed and proteins were subsequently separated via 2-D electrophoresis. After comparison of the serum proteomes from the two groups, protein expression was analyzed. The differentially expressed bands were determined using both matrix-assisted laser ionization time-of-flight (MALDI/TOF) and electrospray ionization quadruple mass spectrometry (MS).
This study identified 361 proteins, the expression of 110 proteins increased, and 87 proteins decreased in the PNES group compared with TLE group. Four separate proteins were finally identified with MALDI/TOF MS analysis. Compared with PNES group, alpha 1-acid glycoprotein, ceruloplasmin, and S100-β were down-regulated and malate dehydrogenase 2 was up-regulated in the serum of TLE patients.
Our results indicated that changes in serum levels of S100-β, ceruloplasmin, alpha 1-acid glycoprotein 1, and malate dehydrogenase 2 after seizure could be introduced as potential markers to differentiate ES from PNES; however, more advanced studies are required to reach a better understanding of the underlying mechanisms.
区分实际的癫痫发作（ESs）与精神性非癫痫发作（PNES）备受关注。本研究比较了被诊断为ESs和PNES患者的血清蛋白质组学。
八名有发作症状的患者（4名PNES患者和4名颞叶癫痫（TLE）患者）纳入了这项对比研究。在发作后的第一个小时内采集静脉血样。采用标准蛋白质纯化技术，随后通过二维电泳分离蛋白质。比较两组血清蛋白质组后，分析蛋白质表达情况。使用基质辅助激光电离飞行时间质谱（MALDI/TOF）和电喷雾电离四极质谱（MS）确定差异表达条带。
本研究鉴定出361种蛋白质，与TLE组相比，PNES组中110种蛋白质表达增加，87种蛋白质表达减少。最终通过MALDI/TOF MS分析鉴定出四种不同的蛋白质。与PNES组相比，TLE患者血清中的α1-酸性糖蛋白、铜蓝蛋白和S100-β下调，而苹果酸脱氢酶2上调。
我们的结果表明，发作后血清中S100-β、铜蓝蛋白、α1-酸性糖蛋白1和苹果酸脱氢酶2水平的变化可作为区分ES与PNES的潜在标志物；然而，需要更深入的研究来更好地理解其潜在机制。
