Postgraduate in Biosciences, University of Guanajuato, Irapuato, Guanajuato, Mexico.
Department of Veterinary and Zootechnics, University of Guanajuato, Irapuato, Guanajuato, Mexico.
Chemosphere. 2020 Aug;253:126658. doi: 10.1016/j.chemosphere.2020.126658. Epub 2020 Mar 31.
The objective of the present study was to determine the susceptibility of entomopathogenic nematodes to ivermectin and thiabendazole. Soil samples collected from the municipalities of Irapuato and León, Guanajuato, Mexico, were obtained, from which the entomopathogenic nematodes (EPNs) of the Steinernematidae and Heterorhabditidae families were isolated. The samples were classified from livestock and nonlivestock soils, and the susceptibility of EPNs to anthelmintics was determined with the larval motility assay (LMA, 24 h) and the larval migration inhibition assay (LMI assay, 48 h). Sterile distilled water (T1) and treatments with 1% ivermectin diluted in 5% DMSO (dimethyl sulfoxide) (T2) and 5% thiabendazole diluted in 5% DMSO (T3) were applied to infective juvenile larvae. Analysis of variance was performed with a factorial design and Tukey's test at 0.05 probability. In addition, different concentrations of ivermectin (0.1, 0.5, 1, 1.2, 1.5, and 2 μg) and thiabendazole (1, 5, 10, 12, 15, and 20 mg) were evaluated to perform a Probit analysis to determine their LC50. All strains of EPNs were susceptible to ivermectin in both the LMA and LMI assay. The results show that EPNs are susceptible to ivermectin and thiabendazole, and the degree depends on the type of test performed, the chemical product used, and the origin of the strain of EPN.
本研究的目的是确定昆虫病原线虫对伊维菌素和噻苯达唑的敏感性。从墨西哥瓜纳华托州伊拉普阿托和莱昂市采集土壤样本，从中分离出斯氏线虫科和异小杆线虫科的昆虫病原线虫（EPN）。将样本分为来自家畜和非家畜土壤，并使用幼虫运动检测（LMA，24 小时）和幼虫迁移抑制检测（LMI 检测，48 小时）来确定 EPN 对驱虫剂的敏感性。应用无菌蒸馏水（T1）和用 5%二甲基亚砜（DMSO）稀释的 1%伊维菌素（T2）以及用 5% DMSO 稀释的 5%噻苯达唑（T3）处理感染性幼虫。采用析因设计和 Tukey 检验进行方差分析，概率水平为 0.05。此外，评估了不同浓度的伊维菌素（0.1、0.5、1、1.2、1.5 和 2μg）和噻苯达唑（1、5、10、12、15 和 20mg），以进行 Probit 分析来确定其 LC50。所有 EPN 菌株在 LMA 和 LMI 检测中均对伊维菌素敏感。结果表明，EPN 对伊维菌素和噻苯达唑敏感，其程度取决于所进行的测试类型、使用的化学产品以及 EPN 菌株的来源。
