Beugnet F, Kerboeuf D, Nicolle J C, Soubieux D
Service de Parasitologie, Ecole Nationale Vétérinaire, Marcy l'Etoile, France.
Vet Parasitol. 1996 May;63(1-2):83-94. doi: 10.1016/0304-4017(95)00879-9.
Ultrastructural changes induced in vitro by thiabendazole, levamisole, pyrantel and ivermectin in the free living larval stages of two trichostrongyles (Heligmosomoides polygyrus and Haemonchus contortus) were analysed. The observed damage for each anthelmintic is related to the known mode of action and compared to the damage commonly described in adults. The advantage of using larvae to study the effects of anthelmintics on the fine structure of nematodes rather than adults is described. Thiabendazole induced alteration of the cellular organization especially epithelial cells of the digestive tract. Changes in mitochondria were also seen. Levamisole caused contraction of muscle fibres whereas no specific lesions were observed with pyrantel. Ivermectin caused an hypertrophy of muscular groups. The interest of such a technique in research on the modes of action of anthelmintics is emphasized.
分析了噻苯达唑、左旋咪唑、噻嘧啶和伊维菌素在体外对两种毛圆线虫（多枝细颈线虫和捻转血矛线虫）自由生活幼虫阶段所诱导的超微结构变化。每种驱虫药所观察到的损伤与已知作用模式相关，并与成虫中通常描述的损伤进行比较。描述了使用幼虫而非成虫来研究驱虫药对线虫精细结构影响的优势。噻苯达唑引起细胞组织尤其是消化道上皮细胞的改变，还可见线粒体变化。左旋咪唑导致肌纤维收缩，而噻嘧啶未观察到特异性损伤。伊维菌素引起肌肉群肥大。强调了这种技术在驱虫药作用模式研究中的意义。
