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猪孤雌生殖胎儿早期妊娠的发育和退化特征

Developmental and Degenerative Characterization of Porcine Parthenogenetic Fetuses during Early Pregnancy.

作者信息

Hwang In-Sul, Park Mi-Ryung, Lee Hae-Sun, Kwak Tae-Uk, Son Hwa-Young, Kang Jong-Koo, Lee Jeong-Woong, Lee Kichoon, Park Eung-Woo, Hwang Seongsoo

机构信息

Animal Biotechnology Division, National Institute of Animal Science, Rural Development Administration, Wanju, Jeonbuk 55365, Korea.

College of Veterinary Medicine, Chungnam National University, Daejeon 34134, Korea.

出版信息

Animals (Basel). 2020 Apr 4;10(4):622. doi: 10.3390/ani10040622.

DOI:10.3390/ani10040622
PMID:32260352
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7222715/
Abstract

The difference between early pregnancy and delivery rate is quite large in assisted reproduction techniques (ARTs), including animal cloning. However, it is not clear why the implanted fetuses aborted after the early pregnancy stage. In the present study, we tried to evaluate the developmental and morphological characteristics of porcine parthenogenetically activated (PA) embryos or fetuses by electric stimulation during the early pregnancy period. The implanted PA and artificially inseminated (AI) embryos and fetuses were collected at day 26 and 35 after embryo transfer, respectively. The developmental and morphological parameters in the PA embryos at day 26 were similar to the AI embryos. The size, weight, formation of major organs, and apoptotic cells were not statistically different in both embryos at day 26. However, the PA fetuses at day 35 showed ceased fetal development and degenerated with abnormal morphologies in their organs. The day 35 PA fetuses showed significantly higher apoptotic cells and lower methylation status in three differentially methylated regions of the H19 gene compared to their comparators. Therefore, the normal development of PA embryos and fetuses during early gestation could lead to these pregnancies being misinterpreted as normal and become one of the main reasons for the gap between early pregnancy and delivery rate.

摘要

在包括动物克隆在内的辅助生殖技术（ARTs）中，早期妊娠与分娩率之间的差异相当大。然而，尚不清楚为什么植入的胎儿在妊娠早期阶段后会流产。在本研究中，我们试图通过电刺激评估猪孤雌生殖激活（PA）胚胎或胎儿在妊娠早期的发育和形态特征。分别在胚胎移植后第26天和第35天收集植入的PA胚胎和人工授精（AI）胚胎及胎儿。第26天PA胚胎的发育和形态参数与AI胚胎相似。在第26天，两个组胚胎的大小、重量、主要器官形成和凋亡细胞在统计学上无差异。然而，第35天的PA胎儿显示胎儿发育停止，并伴有器官形态异常的退变。与对照组相比，第35天的PA胎儿在H19基因的三个差异甲基化区域显示出明显更高的凋亡细胞和更低的甲基化状态。因此，PA胚胎和胎儿在妊娠早期的正常发育可能导致这些妊娠被误判为正常妊娠，并成为早期妊娠与分娩率之间差距的主要原因之一。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/43df/7222715/3bbd1d6e4373/animals-10-00622-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/43df/7222715/e818bdb20ef5/animals-10-00622-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/43df/7222715/c724348ce67a/animals-10-00622-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/43df/7222715/86bab076f5c3/animals-10-00622-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/43df/7222715/7cb433a169c1/animals-10-00622-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/43df/7222715/6e8528fc35fc/animals-10-00622-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/43df/7222715/3bbd1d6e4373/animals-10-00622-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/43df/7222715/e818bdb20ef5/animals-10-00622-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/43df/7222715/c724348ce67a/animals-10-00622-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/43df/7222715/86bab076f5c3/animals-10-00622-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/43df/7222715/7cb433a169c1/animals-10-00622-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/43df/7222715/6e8528fc35fc/animals-10-00622-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/43df/7222715/3bbd1d6e4373/animals-10-00622-g006.jpg

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