Zhou Panyu, Xia Yan, Wang Jing, Liang Chong, Yu Long, Tang Wei, Gu Shen, Xu Shuogui
Changhai Hospital, Department of Orthopedics, the Second Military Medical University, 168 Changhai Road, Shanghai 200433, China.
J Mater Chem B. 2013 Feb 7;1(5):685-692. doi: 10.1039/c2tb00102k. Epub 2012 Nov 28.
Infection and its complications are one of the greatest threats to the health of orthopedic patients. Mesoporous bioglass (MBG) scaffolds are characterized by well-ordered, three-dimensional, nanometer-sized mesoporous structures, which facilitate the adhesion of hydroxyapatite and the loading of drugs. MBG has been widely used as a new-generation biomaterial in bone tissue engineering. However, MBG is very brittle and lacks antibacterial activity. This limits its applications in the treatment of bone defects, especially large bone defects complicated by infection. In order to dispel these disadvantages, a novel hydroxypropyltrimethyl ammonium chloride chitosan (HACC) and a class of prolamine proteins found in maize, Zeins, were here used to modify the traditional MBG scaffolds. Two new types of scaffold, MBG-HACC scaffolds and MBG-HACC-Zein scaffolds, were made. Transmission electron microscopy (TEM), small angle X-ray diffraction (SAXRD), and Barrett-Joyner-Halenda (BJH) were used to analyze the surface properties of these MBG scaffolds. Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), scanning electron microscopy (SEM), mechanical experiments, and synchrotron radiation microcomputer tomography (SRμCT) were used to compare the features of the traditional and modified scaffolds and to analyze the mineralization of the scaffold after being soaked in simulated body fluid (SBF). Confocal laser scanning microscopy (CLSM) was used to compare the antibacterial properties and biocompatibility of the scaffolds at various points in time. The current study demonstrates that all these prepared MBG scaffolds possessed well-ordered, three-dimensional, nanometer-sized mesoporous structures and that HACC-Zein-modified MBG scaffolds are characterized by strong bioactivity and by effective, prolonged antibacterial activity. Finally, biocompatibility was demonstrated by studying the in vitro proliferation and viability of human mesenchymal stem cells (hMSCs).
感染及其并发症是骨科患者健康面临的最大威胁之一。介孔生物玻璃（MBG）支架具有有序的三维纳米级介孔结构，有利于羟基磷灰石的附着和药物负载。MBG作为新一代生物材料已在骨组织工程中广泛应用。然而，MBG非常脆且缺乏抗菌活性。这限制了其在骨缺损治疗中的应用，尤其是伴有感染的大骨缺损。为了消除这些缺点，本文使用一种新型的羟丙基三甲基氯化铵壳聚糖（HACC）和一类在玉米中发现的醇溶蛋白（玉米醇溶蛋白）对传统的MBG支架进行改性。制备了两种新型支架，即MBG - HACC支架和MBG - HACC - 玉米醇溶蛋白支架。采用透射电子显微镜（TEM）、小角X射线衍射（SAXRD）和巴雷特 - 乔伊纳 - 哈伦达（BJH）法分析这些MBG支架的表面性质。利用傅里叶变换红外光谱（FTIR）、X射线衍射（XRD）、扫描电子显微镜（SEM）、力学实验和同步辐射微计算机断层扫描（SRμCT）比较传统支架和改性支架的特性，并分析支架在模拟体液（SBF）中浸泡后的矿化情况。使用共聚焦激光扫描显微镜（CLSM）比较不同时间点支架的抗菌性能和生物相容性。当前研究表明，所有制备的MBG支架均具有有序的三维纳米级介孔结构，且HACC - 玉米醇溶蛋白改性的MBG支架具有很强的生物活性和有效、持久的抗菌活性。最后，通过研究人间充质干细胞（hMSCs）的体外增殖和活力证明了生物相容性。
