Liu Junqing, Song Tao, Yang Qiuhua, Tan Jian, Huang Dinghai, Chang Jin
Institute of Nanobiotechnology, School of Materials Science and Engineering, Tianjin University, Tianjin Key Laboratory of Composites and Functional Materials, Tianjin, 300072, P. R. China.
J Mater Chem B. 2013 Feb 28;1(8):1156-1163. doi: 10.1039/c2tb00162d. Epub 2013 Jan 8.
For the application of biological quantitative probes, how to ameliorate chemical sensitivity and instability of quantum dots (QDs) under different environments is a longstanding issue. Here, silica and poly(EGDMA-co-MAA) as inert materials, ultrastable QD/SiO/poly(EGDMA-co-MAA) fluorescent nanoprobes with a tri-layer core-shell structure were successfully prepared by distillation precipitation polymerization. The QD/SiO/poly(EGDMA-co-MAA) demonstrated ultra-high chemical stability due to the synergistic combination of silica and cross-linked polymer stabilizing the fluorescence intensity of QDs under harsh chemical environments, including strong acidic solutions, which is unavailable for any of the current encapsulation technologies used alone. Immunochromatography test strips (ICTS) for the detection of human chorionic gonadotrophin (HCG) antigen was developed by using QD/SiO/poly(EGDMA-co-MAA) as fluorescent nanoprobes. The results showed admirable reliability and sensitivity in antigen detection. What's more, reliable quantitative detection based on QD/SiO/poly(EGDMA-co-MAA) was realized. We expect the ultrastable QDs will open up exciting opportunities in accurate quantitative analysis and imaging.
对于生物定量探针的应用而言，如何改善量子点（QDs）在不同环境下的化学敏感性和不稳定性是一个长期存在的问题。在此，以二氧化硅和聚（EGDMA - co - MAA）作为惰性材料，通过蒸馏沉淀聚合法成功制备了具有三层核壳结构的超稳定QD/SiO/聚（EGDMA - co - MAA）荧光纳米探针。由于二氧化硅和交联聚合物的协同作用稳定了量子点在包括强酸性溶液在内的苛刻化学环境下的荧光强度，QD/SiO/聚（EGDMA - co - MAA）表现出超高的化学稳定性，这是目前单独使用的任何封装技术都无法实现的。通过使用QD/SiO/聚（EGDMA - co - MAA）作为荧光纳米探针，开发了用于检测人绒毛膜促性腺激素（HCG）抗原的免疫层析试纸条（ICTS）。结果表明，该试纸条在抗原检测中具有出色的可靠性和灵敏度。此外，基于QD/SiO/聚（EGDMA - co - MAA）实现了可靠的定量检测。我们期望这种超稳定的量子点将在精确的定量分析和成像方面带来令人兴奋的机遇。
